Acquiring DNA from wild bats (Mammalia: Chiroptera) is typically undertaken utilizing highly invasive (but non-lethal) sampling techniques comprising wing biopsies and occasional blood samples. While non-invasive sampling is possible through the extraction of DNA from faecal samples, it is not always possible to acquire samples from individual bats whilst conducting fieldwork, and as such, this method is primarily applicable to roost occupancy identification. Similarly, wing swabbing is liable to cross-contamination from roost mates. Here we present the first use of oral (buccal) swabbing for successful, species-resolution DNA sequencing of Vespertilionidae and Rhinolophidae in 10 bat species (nine Vespertilionidae and one Rhinolophidae) from the UK.

从野生蝙蝠（哺乳动物：翼手目）获取 DNA 通常采用高度侵入性（但非致命）的采样技术，包括翅膀活检和偶尔的血液样本。虽然通过从粪便样本中提取 DNA 可以实现非侵入性采样，但在进行实地工作时并不总是能够从单个蝙蝠身上获取样本，因此，该方法主要适用于栖息地识别。同样，翅膀拭子很容易受到栖息伙伴的交叉污染。在这里，我们首次使用口腔（口腔）拭子对来自英国的 10 种蝙蝠（九种蝙蝠科和一种犀牛科）进行成功的物种分辨率 DNA 测序。