CD13 (APN) is an Alanyl-Aminopeptidase with diverse functions. The role of CD13 for gliomas is still unknown. In this study, data of glioma patients obtained by TCGA and CGGA databases were used to evaluate the survival rate and prognostic value of CD13 expression level. Protein expression of CD13 was confirmed by immunofluorescence staining of fresh patient tissues. Eight human glioblastoma cell lines were studied by RT-PCR, Western Blot, immunofluorescence staining and flow cytometry to define CD13 expression. Cell lines with different CD13 expression status were treated with a CD13 inhibitor, bestatin, and examined by MTT, scratch and colony formation assaysas well as by apoptosis assay and Western Blots. Bioinformatics analysis indicated that patients with high expression of CD13 had poor survival and prognosis. Additionally, CD13 protein expression was positively associated with clinical malignant characteristics. Investigated glioblastoma cell lines showed distinct expression levels and subcellular localization of CD13 with intracellular enrichment. Bestatin treatment reduced proliferation, migration and colony formation of glioma cells in a CD13-dependent manner while apoptosis was increased. In summary, CD13 has an impact on glioma patient survival and is important for the main function of specific glioma cells.

中文翻译：

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CD13 表达影响神经胶质瘤患者的生存并影响体外人胶质母细胞瘤细胞系的关键功能

CD13 (APN) 是一种具有多种功能的丙氨酰氨基肽酶。 CD13 对神经胶质瘤的作用尚不清楚。本研究利用TCGA和CGGA数据库获得的胶质瘤患者数据来评估CD13表达水平的生存率和预后价值。通过新鲜患者组织的免疫荧光染色证实了 CD13 的蛋白表达。通过 RT-PCR、Western Blot、免疫荧光染色和流式细胞术研究了八种人胶质母细胞瘤细胞系，以确定 CD13 的表达。用 CD13 抑制剂 bestatin 处理具有不同 CD13 表达状态的细胞系，并通过 MTT、划痕和集落形成测定以及细胞凋亡测定和蛋白质印迹进行检查。生物信息学分析表明CD13高表达的患者生存和预后较差。此外，CD13蛋白表达与临床恶性特征呈正相关。研究的胶质母细胞瘤细胞系显示出 CD13 的不同表达水平和亚细胞定位以及细胞内富集。 Bestatin治疗以CD13依赖性方式减少神经胶质瘤细胞的增殖、迁移和集落形成，同时增加细胞凋亡。总之，CD13对神经胶质瘤患者的生存有影响，并且对于特定神经胶质瘤细胞的主要功能很重要。