Glehnia littoralis, a medicinal herb employed in traditional practices for alleviating fatigue, cough, and a dry throat, is recognized for its beneficial properties due to a diverse array of active compounds found in its extracts. For example, the G. littoralis roots (Radix Glehniae) mainly contain coumarins and phenolic acids, serving as the primary focus of this study. Despite the widespread use of the tools in various industries and the development of multiple analytical methods for their examination, the edible aerial parts have industrial potential, and there is currently no analytical method available to identify their key components. In this study, a high-performance liquid chromatography method combined with diode array detection (HPLC–DAD) was developed to simultaneously detect 16 phenolic compounds previously reported to be present in the edible aerial parts of G. littoralis. The proposed approach included using gradient elution to change the solvent system from water/acetonitrile to water/methanol. Furthermore, the method validation was conducted, assessing its linearity, limit of detection, limit of quantification, precision, accuracy, and recovery, all of which demonstrated satisfactory results. Subsequently, the developed method was applied to quantify the phenolic compounds in various G. littoralis samples obtained from different organs, solvent extraction processes, and processing methods. Moreover, the online HPLC-ABTS (2,2ʹ-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) assay was used to evaluate the antioxidant capacities of individual constituents, identifying four important antioxidants and estimate the overall antioxidant capacity of the G. littoralis extract.

中文翻译：

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高效液相色谱二极管阵列检测（HPLC-DAD）方法的开发和应用，同时定量北沙参地上部分中的酚类化合物

北沙参是一种在传统实践中用于缓解疲劳、咳嗽和喉咙干燥的药草，因其提取物中含有多种活性化合物而因其有益特性而闻名。例如，北沙参根（Radix Glehniae）主要含有香豆素和酚酸，是本研究的主要关注点。尽管这些工具在各个行业中得到广泛使用，并且开发了多种检测分析方法，但可食用地上部分仍具有工业潜力，目前还没有可用的分析方法来鉴定其关键成分。在本研究中，开发了一种结合二极管阵列检测（HPLC-DAD）的高效液相色谱方法，可同时检测先前报道的北海棉食用地上部分中存在的 16 种酚类化合物。所提出的方法包括使用梯度洗脱将溶剂系统从水/乙腈更改为水/甲醇。此外，还进行了方法验证，评估了其线性、检出限、定量限、精密度、准确度和回收率，所有这些都显示出令人满意的结果。随后，应用所开发的方法对从不同器官、溶剂提取过程和加工方法获得的各种北海螟样品中的酚类化合物进行定量。此外，在线HPLC-ABTS（2,2ʹ-azino-bis（3-乙基苯并噻唑啉-6-磺酸）测定法用于评估单个成分的抗氧化能力，识别四种重要的抗氧化剂并估计G的整体抗氧化能力.北沙参提取物。