We developed a high-throughput, unbiased strategy for the identification of endogenous biomolecular condensates by merging cell volume compression, sucrose density gradient centrifugation and quantitative mass spectrometry. We demonstrated the performance of this strategy by identifying both global condensate proteins and those responding to specific biological processes on a proteome-wide scale.

中文翻译：

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识别细胞中的相分离生物分子凝聚体

我们开发了一种高通量、无偏见的策略，通过结合细胞体积压缩、蔗糖密度梯度离心和定量质谱来鉴定内源性生物分子凝聚物。我们通过鉴定全局凝聚蛋白和在蛋白质组范围内对特定生物过程做出反应的蛋白，证明了该策略的性能。