This study presents a global strategy for the transsulfuration of intracellular thiols (RSH) to persulfides (RSSH). Thiiranes comprising fluorenyl/diphenyl and malonate ester moieties directly convert intercellular RSH to low-molecular-weight RSSH in cells. The efficiency of transsulfuration is determined by counting the number of olefins produced as byproducts, providing ratiometric signals for the corresponding persulfide production. Specifically, the direct and rapid protein S-persulfidation by thiirane is validated. Thiiranes are expected to play a crucial role in the study of sulfur signaling.

中文翻译：

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硫杂环丁烷：S-过硫化智能分子

本研究提出了细胞内硫醇 (RSH) 转硫为过硫化物 (RSSH) 的全局策略。包含芴基/二苯基和丙二酸酯部分的硫杂丙环直接将细胞间RSH转化为细胞内的低分子量RSSH。转硫效率是通过计算副产物产生的烯烃数量来确定的，为相应的过硫化物产量提供比率信号。具体来说，验证了硫杂丙环直接快速的蛋白质 S-过硫化作用。硫杂环丁烷有望在硫信号研究中发挥至关重要的作用。