Background Genetic determinants are known to promote antibiotic resistance through horizontal gene transfer. Methods We molecularly characterized integrons, plasmid replicon types and metallo-β-lactamase-encoding genes of 38 Pseudomonas aeruginosa strains isolated from clinical samples. Results The P. aeruginosa isolates displayed high resistance (97.4%) to β-lactams. Seventeen (44.74%) of them possessed plasmids. Of the 17 isolates that possessed plasmids, 11 (64.7%) of them harboured IncFIA plasmid replicon type, while 6 (35.3%), 5 (29.4%) and 5 (29.4%) were of the IncFIB, IncF and IncW types, respectively. The intI1 gene was detected in 19 (50%) of the isolates. The blaNDM-A, blaNDM-B and blaVIM genes were detected in 14 (35.9%), 4 (10.3%) and 5 (12.8%) of the isolates, respectively. Conclusions High resistance to β-lactams was observed among P. aeruginosa strains of clinical origin in this study. They possessed transmissible genetic elements indicating the potential for continuous dissemination, thus continuous surveillance is advocated.

中文翻译：

---

尼日利亚奥贡州三级医院临床样本中分离的铜绿假单胞菌的抗菌素耐药性遗传决定因素和敏感性分析

背景已知遗传决定因素通过水平基因转移促进抗生素耐药性。方法 我们对从临床样本中分离的 38 株铜绿假单胞菌菌株的整合子、质粒复制子类型和金属-β-内酰胺酶编码基因进行了分子特征分析。结果 铜绿假单胞菌对β-内酰胺类药物表现出较高的耐药性（97.4%）。其中17人（44.74%）拥有质粒。 17个携带质粒的分离株中，11个（64.7%）为IncFIA型质粒复制子，6个（35.3%）、5个（29.4%）和5个（29.4%）分别为IncFIB、IncF和IncW型。 。在 19 个（50%）分离株中检测到了 intI1 基因。 blaNDM-A、blaNDM-B 和 blaVIM 基因分别在 14 个（35.9%）、4 个（10.3%）和 5 个（12.8%）分离株中检测到。结论 本研究中临床来源的铜绿假单胞菌菌株对β-内酰胺类药物具有较高的耐药性。它们具有可传播的遗传因素，表明有持续传播的潜力，因此提倡持续监测。