A novel spectrofluorimetric method has been developed for determination of antazoline (ANT) and tetryzoline (TET) in their pharmaceutical formulation. A combined application of synchronous spectrofluorimetry and second derivative mathematical treatment was developed. The proposed method depends on reacting the cited drugs with dansyl chloride (DNS‐Cl) being a suitable derivatizing agent generating highly fluorescent derivatives measured at emission wavelengths of 703.0 and 642.0 nm after excitation wavelengths of 350.0 and 320.0 nm for ANT and TET, respectively. The joint use of synchronous spectrofluorimetry with second derivative mathematical treatment is for the first time to be developed and optimized in aid of using fluorescence data manager software generating second derivative peak amplitudes at 556.5 nm for ANT and 516.7 nm for TET. Linear responses have been represented over a wide range of concentration (0.5–12.0 μg/mL for ANT and 0.5–10.0 μg/mL for TET). Additionally, statistical comparison of the developed method with the official ones has been carried out where no significant difference was found. Additionally, greenness profile assessment was accomplished by means of four metric tools. Indeed, the method developed is found to be precise, sensitive, and discriminating to assess the cited drugs for regular analysis.

中文翻译：

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一种同时测定眼科制剂中安他唑啉和四唑啉的新型绿色荧光分光光度法

开发了一种新型荧光分光光度法，用于测定药物制剂中的安他唑啉 (ANT) 和四唑啉 (TET)。开发了同步荧光光度法和二阶导数数学处理的组合应用。所提出的方法取决于所引用的药物与丹磺酰氯（DNS-Cl）的反应，丹磺酰氯是一种合适的衍生剂，产生高荧光衍生物，在 ANT 和 TET 的激发波长分别为 350.0 和 320.0 nm 后，在 703.0 和 642.0 nm 的发射波长处测量。首次开发和优化同步分光荧光测定法与二阶导数数学处理的联合使用，以帮助使用荧光数据管理软件生成 ANT 556.5 nm 处的二阶导数峰值幅度和 TET 516.7 nm 处的二阶导数峰值幅度。线性响应在很宽的浓度范围内表现出来（ANT 为 0.5–12.0 μg/mL，TET 为 0.5–10.0 μg/mL）。此外，对所开发的方法与官方方法进行了统计比较，未发现显着差异。此外，绿度概况评估是通过四种度量工具完成的。事实上，我们发现所开发的方法精确、灵敏且具有辨别力，可以评估所引用的药物进行定期分析。