Enteric methane (CH) produced by ruminant livestock is a potent greenhouse gas and represents significant energy loss for the animal. The novel application of oxidising compounds as antimethanogenic agents with future potential to be included in ruminant feeds, was assessed across two separate experiments in this study. Low concentrations of oxidising agents, namely urea hydrogen peroxide (UHP) with and without potassium iodide (KI), and magnesium peroxide (MgO), were investigated for their effects on CH production, total gas production (TGP), volatile fatty acid (VFA) profiles, and nutrient disappearance using the rumen simulation technique. In both experiments, the diet consisted of 50:50 grass silage:concentrate on a dry matter basis. Treatment concentrations were based on the amount of oxygen delivered and expressed in terms of fold concentration. In Experiment 1, four treatments were tested (Control, 1× UHP + KI, 1× UHP, and 0.5× UHP + KI), and six treatments were assessed in Experiment 2 (Control, 0.5× UHP + KI, 0.5× UHP, 0.25× UHP + KI, 0.25× UHP, and 0.12× MgO). All treatments in this study had a reducing effect on CH parameters. A dose-dependent reduction of TGP and CH parameters was observed, where treatments delivering higher levels of oxygen resulted in greater CH suppression. 1× UHP + KI reduced TGP by 28 % ( = 0.611), CH% by 64 % ( = 0.075) and CH mmol/g digestible organic matter by 71 % ( = 0.037). 0.12× MgO reduced CH volume by 25 % ( > 0.05) without affecting any other parameters. Acetate-to-propionate ratios were reduced by treatments in both experiments ( < 0.01). Molar proportions of acetate and butyrate were reduced, while propionate and valerate were increased in UHP treatments. High concentrations of UHP affected the degradation of neutral detergent fibre in the forage substrate. Future work should investigate alternative slow-release oxygen sources aimed at prolonging CH suppression.

中文翻译：

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新型氧化饲料添加剂利用瘤胃模拟技术减少体外甲烷排放

反刍牲畜产生的肠内甲烷 (CH) 是一种强效温室气体，对动物来说意味着巨大的能量损失。本研究通过两个独立的实验对氧化化合物作为抗致死剂的新应用进行了评估，该化合物未来有可能包含在反刍动物饲料中。研究了低浓度氧化剂，即含或不含碘化钾 (KI) 的过氧化氢尿素 (UHP) 和过氧化镁 (MgO) 对 CH 产量、总气体产量 (TGP)、挥发性脂肪酸 (VFA) 的影响）概况，以及使用瘤胃模拟技术的营养物消失。在这两个实验中，日粮由 50:50 的草青贮饲料和浓缩饲料（以干物质计）组成。处理浓度基于输送的氧气量并以浓度倍数表示。在实验1中，测试了四种处理（对照、1×UHP+KI、1×UHP和0.5×UHP+KI），在实验2中评估了六种处理（对照、0.5×UHP+KI、0.5×UHP、 0.25× UHP + KI、0.25× UHP 和 0.12× MgO)。本研究中的所有处理对 CH 参数都有降低作用。观察到 TGP 和 CH 参数呈剂量依赖性降低，其中提供更高水平氧气的治疗导致更大的 CH 抑制。 1× UHP + KI 使 TGP 降低 28 % (= 0.611)，CH% 降低 64 % (= 0.075)，CH mmol/g 可消化有机物降低 71 % (= 0.037)。 0.12× MgO 使 CH 体积减少 25% (> 0.05)，而不影响任何其他参数。两个实验中的处理均降低了乙酸与丙酸的比率（< 0.01）。在 UHP 处理中，乙酸盐和丁酸盐的摩尔比例降低，而丙酸盐和戊酸盐的摩尔比例增加。高浓度的超高压会影响饲料基质中中性洗涤纤维的降解。未来的工作应该研究旨在延长 CH 抑制的替代缓释氧源。