The pharmacokinetics of florfenicol (FFC) in green sea and hawksbill sea turtles were evaluated following intramuscular (i.m.) administration at two different dosages of 20 or 30 mg/kg body weight (b.w.). This study (longitudinal design) used 5 green sea and 5 hawksbill sea turtles for the two dosages. Blood samples were collected at assigned times up to 168 h. FFC plasma samples were analyzed using validated high‐performance liquid chromatography equipped with diode array detection. The pharmacokinetic analysis was performed using a non‐compartment approach. The FFC plasma concentrations increased with the dosage. The elimination half‐life was similar between the treatment groups (range 19–25 h), as well as the plasma protein binding (range 18.59%–20.65%). According to the surrogate PK/PD parameter (T > MIC, 2 μg/mL), the 20 and 30 mg/kg dosing rates should be effective doses for susceptible bacterial infections in green sea and hawksbill sea turtles.

中文翻译：

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氟苯尼考在绿海龟（Chelonia mydas）和玳瑁海龟（Eretmochelys imbricata）肌肉注射后的药代动力学特征

以 20 或 30 mg/kg 体重 (bw) 两种不同剂量肌肉注射 (im) 后，评估了氟苯尼考 (FFC) 在绿海龟和玳瑁海龟中的药代动力学。本研究（纵向设计）使用 5 只绿海龟和 5 只玳瑁海龟进行两种剂量的治疗。在指定时间采集血样，最长可达 168 小时。使用配备二极管阵列检测器的经过验证的高效液相色谱法对 FFC 血浆样品进行分析。使用非房室方法进行药代动力学分析。 FFC血浆浓度随剂量增加而增加。治疗组之间的消除半衰期（范围 19-25 小时）以及血浆蛋白结合（范围 18.59%-20.65%）相似。根据替代 PK/PD 参数（T > MIC，2 μg/mL），20 和 30 mg/kg 剂量率应是绿海龟和玳瑁海龟易感细菌感染的有效剂量。