CircRNAs represent a new class of non‐coding RNAs which show aberrant expression in diverse cancers, such as gastric cancer (GC). circSTRBP, for instance, is suggested to be overexpressed in GC cells and tissues. However, the biological role of circSTRBP in the progression of GC and the potential mechanisms have not been investigated. circSTRBP levels within GC cells and tissues were measured by RT‐qPCR. The stability of circSTRBP was assessed by actinomycin D and Ribonuclease R treatment. Cell proliferation, migration, invasion and in vitro angiogenic abilities after circSTRBP knockdown were analysed through CCK‐8 assay, transwell culture system and the tube formation assay. The interaction of circSTRBP with the predicted target microRNA (miRNA) was examined by RNA immunoprecipitation and luciferase reporter assays. Xenograft tumour model was established to evaluate the role of exosomal circSTRBP in the tumour formation of GC cells. circSTRBP was upregulated in GC cells and tissues, and there was an increased level of circSTRBP in GC‐derived exosomes. circSTRBP in the exosomes enhanced GC cell growth and migration in vitro, which modulates E2F Transcription Factor 2 (E2F2) expression through targeting miR‐1294 and miR‐593‐3p. Additionally, exosomal circSTRBP promoted the tumour growth of GC cells in the xenograft model. Exosomal circSTRBP is implicated in the progression of GC by modulating the activity of miR‐1294/miR‐593‐3p/E2F2 axis.

中文翻译：

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癌细胞的外泌体 circSTRBP 通过调节 miR-1294/miR-593-3p/E2F2 轴促进胃癌进展

CircRNA 代表了一类新的非编码 RNA，在胃癌 (GC) 等多种癌症中表现出异常表达。例如，circSTRBP 被认为在 GC 细胞和组织中过度表达。然而，circSTRBP在GC进展中的生物学作用及其潜在机制尚未得到研究。通过 RT-qPCR 测量 GC 细胞和组织内的 circSTRBP 水平。通过放线菌素 D 和核糖核酸酶 R 处理评估 circSTRBP 的稳定性。通过CCK-8实验、Transwell培养系统和成管实验分析circSTRBP敲低后的细胞增殖、迁移、侵袭和体外血管生成能力。通过 RNA 免疫沉淀和荧光素酶报告基因检测来检查 circSTRBP 与预测的目标 microRNA (miRNA) 的相互作用。建立异种移植肿瘤模型以评估外泌体circSTRBP在GC细胞肿瘤形成中的作用。 circSTRBP 在 GC 细胞和组织中上调，并且 GC 衍生的外泌体中 circSTRBP 水平升高。外泌体中的 circSTRBP 在体外增强了 GC 细胞的生长和迁移，通过靶向 miR-1294 和 miR-593-3p 调节 E2F 转录因子 2 (E2F2) 的表达。此外，外泌体 circSTRBP 促进异种移植模型中 GC 细胞的肿瘤生长。外泌体 circSTRBP 通过调节 miR-1294/miR-593-3p/E2F2 轴的活性参与 GC 的进展。