Abstract

A major one among bacteria used in the commercial production of enzymes is Bacillus sp.; others include Escherichia sp., Pseudomonas, Proteus, Serratia, and Rhizobium, which also yield an appreciable quantity of enzymes. Biosurfactants naturally play a vital role in the teeming motility of microbes and contribute to cellular physiological procedures of signalling and, differentiation, and biofilm formation. The sourcing and selection of microbes from natural sources have endlessly been shown to be an effective way of identifying isolates of industrial value. The current study aimed to determine the ability of exopolysaccharide-producing bacteria isolated from cassava peel heap to produce some extracellular enzymes and biosurfactants. Thirteen bacteria earlier isolated from cassava peel heaps samples and found to be exopolysaccharide producers were used in this study. The highest xylanase activity was recorded at 40°C by B. amyloliquefaciens (J47). Titratable acid content was highest in peel fermented with isolate Lactobacillus plantarum (J18). In contrast, the pH of fermented peel fell below pH 7, with the lowest being the peel fermented by Pectobacterium carotovorum (J36). Three Bacillus spp. (Bacillus sp. J1, Bacillus subtilis J2, and B. amyloliquefaciens J47) were able to produce all the three enzymes (xylanase, cellulase, and β-glucosidase) assayed for. Bacterial isolates from cassava peel heap can be helpful in many industrial processes with their ability to produce substances of industrial value.

中文翻译：

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从木薯皮堆中分离出的胞外多糖生产细菌生产酶和生物表面活性剂

摘要

用于商业生产酶的细菌中的主要一种是芽孢杆菌属。其他细菌包括埃希氏菌属、假单胞菌属、变形杆菌属、沙雷氏菌属和根瘤菌属，它们也产生大量的酶。生物表面活性剂自然在微生物的丰富运动中发挥着至关重要的作用，并有助于信号传导、分化和生物膜形成的细胞生理过程。从自然资源中采购和选择微生物已被不断地证明是识别具有工业价值的菌株的有效方法。目前的研究旨在确定从木薯皮堆中分离出的胞外多糖产生细菌产生一些胞外酶和生物表面活性剂的能力。本研究使用了早期从木薯皮堆样品中分离出来并被发现是胞外多糖生产者的 13 种细菌。解淀粉芽孢杆菌(J47)在 40°C 时记录到最高木聚糖酶活性。用分离植物乳杆菌(J18)发酵的果皮中可滴定酸含量最高。相比之下，发酵果皮的pH值降至7以下，其中最低的是由胡萝卜果杆菌(J36)发酵的果皮。三种芽孢杆菌属。 （芽孢杆菌 J1、枯草芽孢杆菌 J2 和解淀粉芽孢杆菌J47）能够产生所检测的所有三种酶（木聚糖酶、纤维素酶和 β-葡萄糖苷酶）。从木薯皮堆中分离出来的细菌可以在许多工业过程中发挥作用，因为它们能够生产具有工业价值的物质。