Nonhuman primates are an important experimental model for the development of targeted biological therapeutics because of their immunological closeness to humans. However, there are very few antibody reagents relevant for delineating the different immune cell subsets based on nonhuman primate antigens directly or with cross-reactivity to those in humans. Here, we report specific expression of HLA-DR, PD-1, and CD123 on different circulating immune cell subsets in the peripheral blood that included T cells (CD3+), T cells subsets (CD4+ and CD8+), B cells (CD20+), natural killer (NK) cells (CD3–CD16+), and natural killer T cells (CD3+CD16+) along with different monocyte subsets in squirrel monkey (Saimiri sciureus). We established cross-reactivity of commercial mouse antihuman monoclonal antibodies (mAbs), with these various immune cell surface markers. These findings should aid further future comprehensive understanding of the immune parameters and identification of new biomarkers to significantly improve SQM as a model for biomedical studies.

中文翻译：

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松鼠猴（Saimiri sciureus）免疫细胞上特异性细胞表面标记的鉴定

非人灵长类动物由于其免疫学与人类相似，是开发靶向生物疗法的重要实验模型。然而，很少有抗体试剂能够直接基于非人灵长类动物抗原或与人类抗原具有交叉反应性来描绘不同的免疫细胞亚群。在这里，我们报告了外周血中不同循环免疫细胞亚群上 HLA-DR、PD-1 和 CD123 的特异性表达，包括 T 细胞 (CD3+)、T 细胞亚群（CD4+ 和 CD8+）、B 细胞 (CD20+)、自然杀伤 (NK) 细胞 (CD3–CD16+) 和自然杀伤 T 细胞 (CD3+CD16+) 以及松鼠猴 ( Saimiri sciureus ) 中的不同单核细胞亚群。我们建立了商业小鼠抗人单克隆抗体 (mAb) 与这些不同免疫细胞表面标记的交叉反应性。这些发现应有助于未来进一步全面了解免疫参数和识别新的生物标志物，从而显着改进 SQM 作为生物医学研究的模型。