Abstract

The perplexing issues related to positive surgical margins and the considerable negative consequences associated with systemic chemotherapy have posed ongoing challenges for clinicians, especially when it comes to addressing bladder cancer treatment. The current investigation describes the production of nanocomposites loaded with gemcitabine (GEM) and cisplatin (CDDP) through the utilization of electrospinning technology. In vitro and in vivo studies have provided evidence of the strong effectiveness in suppressing tumor advancement while simultaneously reducing the accumulation of chemotherapy drugs within liver and kidney tissues. Mechanically, the GEM and CDDP-loaded electrospun nanocomposites could effectively eliminate myeloid-derived suppressor cells (MDSCs) in tumor tissues, and recruit CD8⁺ T cells and NKp46⁺ NK cells to kill tumor cells, which can also effectively inhibit tumor microvascular formation. Our investigation into the impact of localized administration of chemotherapy through GEM and CDDP-loaded electrospun nanocomposites on the tumor microenvironment will offer novel insights for tackling tumors.

Graphical abstract

中文翻译：

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负载吉西他滨和顺铂的电纺纳米纤维垫通过改善肿瘤免疫微环境抑制膀胱肿瘤生长

摘要

与阳性手术切缘相关的令人困惑的问题以及与全身化疗相关的相当大的负面后果给临床医生带来了持续的挑战，特别是在解决膀胱癌治疗方面。目前的研究描述了通过利用静电纺丝技术生产负载吉西他滨（GEM）和顺铂（CDDP）的纳米复合材料。体外和体内研究提供了证据，证明其在抑制肿瘤进展同时减少化疗药物在肝和肾组织内的积累方面具有强大的有效性。从机械角度来看，负载GEM和CDDP的电纺纳米复合材料可以有效消除肿瘤组织中的骨髓源性抑制细胞（MDSC），并招募CD8 ⁺ T细胞和NKp46 ⁺ NK细胞杀死肿瘤细胞，还可以有效抑制肿瘤微血管形成。我们通过负载 GEM 和 CDDP 的电纺纳米复合材料进行局部化疗对肿瘤微环境的影响进行研究，将为治疗肿瘤提供新的见解。

图形概要