Abstract

Xyloglucan is a rigid polysaccharide that belongs to the carbohydrate family. This hemicellulose compound has been widely used in biomedical research because of its pseudoplastic, mucoadhesive, mucomimetic, and biocompatibility properties. Xyloglucan is a polyose with no amino groups in its structure, which also limits its range of applications. It is still unknown whether grafting hydrophilic monomers onto xyloglucan can produce derivatives that overcome these shortcomings. This work aimed to prepare the first copolymers in which N-hydroxyethyl acrylamide is grafted onto tamarind xyloglucan by free-radical polymerization. The biocompatibility of these structures in vitro was evaluated using human dermal fibroblasts. Gamma radiation-induced graft polymerization was employed as an initiator by varying the radiation dose from 5–25 kGy. The structure of the graft copolymer, Xy-g-poly(N-hydroxyethyl acrylamide), was verified by thermal analysis, Fourier transform infrared spectroscopy, and nuclear magnetic resonance spectroscopy. The findings indicate that the degree of grafting and the cytotoxicity/viability of the xyloglucan-based copolymer were independent of dose. Notably, the grafted galactoxyloglucan exhibited efficient support for human dermal fibroblasts, showing heightened proliferative capacity and superior migration capabilities compared to the unmodified polymer. This copolymer might have the potential to be used in skin tissue engineering.

中文翻译：

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自由基聚合制备木葡聚糖接枝聚（N-羟乙基丙烯酰胺）共聚物用于人真皮成纤维细胞的体外评价

摘要

木葡聚糖是一种刚性多糖，属于碳水化合物家族。这种半纤维素化合物因其假塑性、粘膜粘附性、粘液模拟性和生物相容性特性而被广泛应用于生物医学研究。木葡聚糖是一种结构中不含氨基的多糖，这也限制了其应用范围。目前尚不清楚将亲水性单体接枝到木葡聚糖上是否可以产生克服这些缺点的衍生物。这项工作旨在制备第一个通过自由基聚合将 N-羟乙基丙烯酰胺接枝到罗望子木葡聚糖上的共聚物。使用人真皮成纤维细胞评估这些结构的体外生物相容性。通过改变 5-25 kGy 的辐射剂量，采用伽马辐射诱导的接枝聚合作为引发剂。通过热分析、傅里叶变换红外光谱和核磁共振光谱验证了接枝共聚物Xy-g-聚(N-羟乙基丙烯酰胺)的结构。研究结果表明，基于木葡聚糖的共聚物的接枝程度和细胞毒性/活力与剂量无关。值得注意的是，与未修饰的聚合物相比，接枝的半乳木葡聚糖表现出对人真皮成纤维细胞的有效支持，显示出更高的增殖能力和优异的迁移能力。这种共聚物可能有用于皮肤组织工程的潜力。