The gene encoding the specific phosphohydrolase LONELY GUY (LOG) plays an important role in the activation of cytokinin and the stress response in plant cells. However, the role of genes in castor bean () has not been reported. In this study, we identified a total of nine members of the gene family in the castor bean genome and investigated the upregulated expression of the gene using transcriptome data analysis. We found that the gene exhibited tissue-specific expression and was activated by polyethylene glycol, NaCl, low temperature, and abscisic acid stress. The subcellular localization results showed that the gene is mainly located in the cytoplasm. Based on phenotypic and physiological indicators, namely root length, peroxidase activity, and malondialdehyde content, overexpression of the gene not only improved the drought resistance, salt tolerance, and cold tolerance of transgenic , but also shortened the dormancy period of the transgenic plants. Transcriptomic sequencing revealed that the overexpression of the gene led to the enrichment of differentially expressed genes in the glutathione metabolism pathway in transgenic . Moreover, the overexpression plants had higher levels of glutathione and a higher GSH/GSSG ratio under stress compared to the wild type. Therefore, we inferred that the gene may be responsible for regulating cell membrane homeostasis by reducing the accumulation of reactive oxygen species through the glutathione pathway. Overall, the overexpression of the gene positively regulated the stress resistance of transgenic This study provides valuable gene resources for breeding stress-tolerant castor bean varieties.

中文翻译：

---

蓖麻和 RcLOG5 中 LOG 家族基因的全基因组分析增强了拟南芥的干旱、盐和冷胁迫耐受性

编码特定磷酸水解酶 LONELY GUY (LOG) 的基因在细胞分裂素的激活和植物细胞的应激反应中发挥着重要作用。然而，基因在蓖麻子中的作用尚未见报道。在这项研究中，我们鉴定了蓖麻子基因组中该基因家族的总共九个成员，并利用转录组数据分析研究了该基因的上调表达。我们发现该基因表现出组织特异性表达，并被聚乙二醇、氯化钠、低温和脱落酸胁迫激活。亚细胞定位结果显示该基因主要定位于细胞质。从根长、过氧化物酶活性、丙二醛含量等表型和生理指标来看，该基因的过表达不仅提高了转基因植物的抗旱、耐盐、耐寒能力，而且缩短了转基因植物的休眠期。转录组测序显示，该基因的过度表达导致转基因中谷胱甘肽代谢途径中差异表达基因的富集。此外，与野生型相比，过表达植物在胁迫下具有更高水平的谷胱甘肽和更高的 GSH/GSSG 比率。因此，我们推测该基因可能通过谷胱甘肽途径减少活性氧的积累来调节细胞膜稳态。总体而言，该基因的过表达对转基因蓖麻子的抗逆性具有正向调节作用，本研究为选育耐逆蓖麻品种提供了宝贵的基因资源。