The chloroplast serves as the primary site of photosynthesis, and its development plays a crucial role in regulating plant growth and morphogenesis. The Pentatricopeptide Repeat Sequence (PPR) proteins constitute a vast protein family that function in the post-transcriptional modification of RNA within plant organelles. In this study, we characterized mutant of rice with pale green leaves (pgl3a). The chlorophyll content of pgl3a at the seedling stage was significantly reduced compared to the wild type (WT). Transmission electron microscopy (TEM) and quantitative PCR analysis revealed that pgl3a exhibited aberrant chloroplast development compared to the wild type (WT), accompanied by significant alterations in gene expression levels associated with chloroplast development and photosynthesis. The Mutmap analysis revealed that a single base deletionin the coding region of Os03g0136700 in pgl3a. By employing CRISPR/Cas9 mediated gene editing, two homozygous cr-pgl3a mutants were generated and exhibited a similar phenotype to pgl3a, thereby confirming that Os03g0136700 was responsible for pgl3a. Consequently, it was designated as OsPGL3A. OsPGL3A belongs to the DYW-type PPR protein family and is localized in chloroplasts. Furthermore, we demonstrated that the RNA editing efficiency of rps8-182 and rpoC2-4106, and the splicing efficiency of ycf3-1 were significantly decreased in pgl3a mutants compared to WT. Collectively, these results indicate that OsPGL3A plays a crucial role in chloroplast development by regulating the editing and splicing of chloroplast genes in rice.

叶绿体是光合作用的主要场所，其发育在调节植物生长和形态建成中起着至关重要的作用。五肽重复序列 (PPR) 蛋白构成了一个庞大的蛋白家族，在植物细胞器内 RNA 的转录后修饰中发挥作用。在这项研究中，我们鉴定了浅绿叶水稻突变体（pgl3a）。与野生型（WT）相比，苗期pgl3a的叶绿素含量显着降低。透射电子显微镜 (TEM) 和定量 PCR 分析表明，与野生型 (WT) 相比，pgl3a表现出异常的叶绿体发育，并伴有与叶绿体发育和光合作用相关的基因表达水平的显着变化。 Mutmap分析显示pgl3a的Os03g0136700编码区存在单碱基缺失。通过使用CRISPR/Cas9介导的基因编辑，产生了两个纯合的cr - pgl3a突变体，并表现出与pgl3a相似的表型，从而证实Os03g0136700负责pgl3a。因此，它被指定为OsPGL3A。OsPGL3A属于 DYW 型 PPR 蛋白家族，定位于叶绿体中。此外，我们证明与WT相比，pgl3a突变体中rps8-182和rpoC2-4106的RNA编辑效率以及ycf3-1的剪接效率显着降低。总的来说，这些结果表明OsPGL3A通过调节水稻叶绿体基因的编辑和剪接，在叶绿体发育中发挥着至关重要的作用。