Obtaining sufficient concentrations of fermentable sugars from the polysaccharides of lignocellulosic materials represents a long-term challenge in achieving sustainable biorefineries. This work aimed to enhance sugar titers by employing high sugar-containing stovers (HSS) and combining its intrinsic soluble sugars with the two insoluble carbohydrates, namely cellulose and hemicellulose. Whole-slurry hydrolysis (WSH) was performed under low-to-high solid conditions using non-detoxified pretreated slurry, which exhibited a decrease in cellulose hydrolysis efficiency (CHE) from 89.08% to 42.1% as the solid loadings increased from 7% to 20%. A further WSH with detoxified slurry showed a significant increase in CHE from 42.1% to 59.52% at 20% solid loading, indicating that inhibitors present in the pretreated slurry had negative effects on CHE. Lower CHE with non-detoxified slurry accompanied by higher losses of cellulase and β-glucosidase activities, particularly at higher solid loadings. Therefore, systematic modification of WSH was performed by incorporating 2% syringic acid during pretreatment and 0.4% polyethylene glycol during saccharification, reformulating enzyme cocktails, and implementing a fed-batch experiment. These modifications resulted in a notable improvement in sugar production under high-solid loading (20%) and non-detoxified conditions, reaching 142.87 g/L total sugars with a CHE of 90.7%. These findings have revealed the potential of integrating soluble sugars with holocellulose for obtaining high-titer fermentable sugars in lignocellulosic biorefineries.

中文翻译：

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为含糖秸秆定制全浆生物加工，通过整合可溶性和不溶性碳水化合物来增加糖产量

从木质纤维素材料的多糖中获得足够浓度的可发酵糖是实现可持续生物精炼厂的长期挑战。这项工作旨在通过使用高含糖秸秆（HSS）并将其固有的可溶性糖与两种不溶性碳水化合物（即纤维素和半纤维素）相结合来提高糖度。使用未解毒的预处理浆料在低至高固体条件下进行全浆料水解 (WSH)，随着固体负载量从 7% 增加到 7%，纤维素水解效率 (CHE) 从 89.08% 降低到 42.1%。 20%。进一步的 WSH 与解毒浆料显示，在 20% 固体负载下，CHE 从 42.1% 显着增加到 59.52%，表明预处理浆料中存在的抑制剂对 CHE 有负面影响。未解毒浆料的 CHE 较低，伴随着纤维素酶和 β-葡萄糖苷酶活性的较高损失，特别是在较高的固体负载下。因此，通过在预处理过程中加入 2% 丁香酸，在糖化过程中加入 0.4% 聚乙二醇，重新配制酶混合物，并进行补料分批实验，对 WSH 进行了系统修饰。这些修改导致在高固含量 (20%) 和非解毒条件下糖产量显着提高，总糖达到 142.87 g/L，CHE 为 90.7%。这些发现揭示了将可溶性糖与全纤维素整合以在木质纤维素生物精炼厂中获得高滴度可发酵糖的潜力。