The application of in-situ aeration technology in landfills has been reported to promote fungal growth, but the community diversity and function of fungi in the aerated landfill system remain unknown. This study firstly investigated an in-situ aerated remediation landfill site to characterize the fungal community diversity in refuse. And to further reveal the fungal involvement in the nitrogen cycling system, laboratory-scale simulated aerated landfill reactors were then constructed. The results in the aerated landfill site showed a significant correlation between fungal community structure and ammonia nitrogen content in the refuse. Dominant fungi in the fungal community included commonly found environmental fungi such as , , , as well as unique fungi in the aerated system like . In the laboratory-scale aerated landfill simulation experiments, the fungal system was constructed using bacterial inhibitor, and nitrogen balance analysis confirmed the significant role of fungal nitrification in the nitrogen cycling process. When ammonia nitrogen was not readily available, fungi converted organic nitrogen to nitrate, serving as the main nitrification mechanism in the system, with a contribution rate ranging from 62.71 % to 100 % of total nitrification. However, when ammonia nitrogen was present in the system, autotrophic nitrification became the main mechanism, and the contribution of fungal nitrification to total nitrification was only 15.96 %. Additionally, fungi were capable of directly utilizing nitrite for nitrate production with a rate of 4.65 mg L d. This research article contributes to the understanding of the importance of fungi in the aerated landfill systems, filling a gap in knowledge.

中文翻译：

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真菌群落多样性及其对原位曝气垃圾填埋场氮循环的贡献：来自现场和实验室研究的见解

据报道，原位曝气技术在垃圾填埋场中的应用可以促进真菌生长，但曝气垃圾填埋场系统中真菌的群落多样性和功能仍不清楚。本研究首先调查了原位曝气修复垃圾填埋场，以表征垃圾中真菌群落的多样性。为了进一步揭示真菌在氮循环系统中的参与，随后建造了实验室规模的模拟曝气垃圾填埋反应器。曝气垃圾填埋场的结果表明，真菌群落结构与垃圾中氨氮含量之间存在显着相关性。真菌群落中的优势真菌包括常见的环境真菌如、、以及曝气系统中特有的真菌。在实验室规模的曝气垃圾填埋场模拟实验中，利用细菌抑制剂构建了真菌系统，氮平衡分析证实了真菌硝化作用在氮循环过程中的重要作用。当氨氮不易获得时，真菌将有机氮转化为硝酸盐，是系统中主要的硝化机制，对总硝化作用的贡献率为62.71%~100%。然而，当系统中存在氨氮时，自养硝化作用成为主要机制，真菌硝化作用对总硝化作用的贡献仅为15.96%。此外，真菌能够直接利用亚硝酸盐生产硝酸盐，产量为 4.65 mg·L·d。这篇研究文章有助于了解真菌在曝气垃圾填埋系统中的重要性，填补了知识空白。