Municipal wastewater (MWW) was treated by a sequential pilot microalgal cultivation process. The cultivation was performed inside a specifically designed low-cost photobioreactor (PBR) system. A microalgal consortium 2:1 was developed using (TS) and sp. (PC) in the first stage and PC:TS (2:1) in the second stage and the nutrient removal efficiency and biomass production and biomolecules production was evaluated and also compared with monoculture in a two-stage sequential cultivation system. This study also investigated the effect of seasonal variations on microalgae growth and MWW treatment. The results showed that mixed microalgal consortium (TS:PC) had higher nutrient removal efficiency, with chemical oxygen demand (COD), total phosphate (TP), and total nitrate (TN) removal efficiencies of 78.50, 84.49, and 84.20%, respectively, and produced a biomass of 2.50 g/L with lipid content of 37.36% in the first stage of cultivation under indoor conditions. In the second stage of indoor cultivation, the PC:TS consortium demonstrated maximum COD, TP, and TN removal efficiencies of 92.49, 94.24, and 94.16%, respectively. It also produced a biomass of 2.65 g/L with a lipid content of 40.67%. Among all the seasonal variations, mass flow analysis indicated that the combination of mixed consortium-based two-stage sequential process during the winter season favored maximum nutrient removal efficiency of TN i.e. 88.54% (84.12 mg/L) and TP i.e., 90.18% (43.29 mg/L), respectively. It also enhanced total biomass production of 49.10 g in 20-L medium, which includes lipid yield ∼15.68 g compared to monoculture i.e., 82.06% (78.70 mg/L) and 82.87% (40.26 mg/L) removal of TN and TP, respectively, and produced biomass 43.60 g with 11.90 g of lipids.

中文翻译：

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用于处理城市废水和生物质生产的亚中试规模序贯微藻联合体培养

通过连续中试微藻培养工艺处理城市废水（MWW）。培养是在专门设计的低成本光生物反应器（PBR）系统内进行的。使用 (TS) 和 sp. 开发了 2:1 的微藻群落。 (PC)在第一阶段，PC:TS (2:1)在第二阶段，评估了养分去除效率、生物量产量和生物分子产量，并与两阶段序贯培养系统中的单一栽培进行比较。这项研究还调查了季节变化对微藻生长和 MWW 处理的影响。结果表明，混合微藻群落（TS:PC）具有较高的养分去除效率，化学需氧量（COD）、总磷（TP）和总硝酸盐（TN）去除效率分别为78.50%、84.49%和84.20%。 ，在室内条件下培养第一阶段产生的生物量为2.50 g/L，脂质含量为37.36%。在室内培养的第二阶段，PC:TS联盟表现出最大的COD、TP和TN去除效率，分别为92.49%、94.24%和94.16%。它还产生了 2.65 g/L 的生物量，脂质含量为 40.67%。在所有季节变化中，质量流量分析表明，冬季基于混合联合体的两阶段序贯过程的组合有利于最大的TN去除效率，即88.54%（84.12 mg/L）和TP，即90.18%（ 43.29 毫克/升），分别。它还将 20 L 培养基中的总生物量产量提高了 49.10 g，其中与单一培养相比，其中脂质产量约 15.68 g，即 TN 和 TP 去除率分别为 82.06% (78.70 mg/L) 和 82.87% (40.26 mg/L)，分别产生了43.60克生物质和11.90克脂质。