The quality of microcystin-LR (MC-LR) standards is frequently low and varies considerably due to the quite complex and challenging isolation and purification of MC-LR from Microcystis aeruginosa lysates. To streamline the way of standard acquisition, we describe an approach that combines rational design and directed chemical evolution for discovering synthetic hydrogel copolymers, which are potential abiotic affinity materials for MC-LR. A small focused polymer library was engineered by incorporating various combinations and ratios of candidate N-acryloyl-(l)-amino acid monomers, including N-acryloyl-glycine (AGly), N-acryloyl-l-phenylalanine (APhe), and N-acryloyl-l-glutamic acid (AGlu), that were selected based on the analysis of structural and physicochemical properties of MC-LR. A hydrogel copolymer AGlu-NP13 (58% AGlu, 40% TBAm, and 2% BIS) that had a high and specific affinity to MC-LR was screened from the polymer library. The hydrogen bond interaction may dominate the AGlu-NP13–MC-LR binding. The external conditions that influence the hydrogen bond interaction, including pH and solvation environments, controlled the catch and release of MC-LR on AGlu-NP13. The uptake of MC-LR is maximum under a saturated ethyl acetate (10%, v/v) solution at pH 3. The bound MC-LR can be completely desorbed from the hydrogel copolymer with a pH 9.5 and 70% methanol aqueous solution. The screened polymer affinity ligand was successfully applied for the isolation and purification of MC-LR from the lyophilized cell extracts of M. aeruginosa 905, providing a potential application of this material in standard preparation, sample pretreatment, and adsorption removal of MC-LR in complicated environmental and biological samples.

中文翻译：

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氢键主导的水凝胶共聚物用于从铜绿微囊藻裂解液中选择性分离和纯化微囊藻毒素-LR

由于从铜绿微囊藻裂解物中分离和纯化 MC-LR 相当复杂且具有挑战性，因此微囊藻毒素-LR (MC-LR) 标准品的质量通常较低且差异很大。为了简化标准采集的方式，我们描述了一种结合合理设计和定向化学进化的方法，用于发现合成水凝胶共聚物，这是 MC-LR 的潜在非生物亲和材料。通过合并候选N-丙烯酰-( l )-氨基酸单体的各种组合和比例，设计了一个小型集中聚合物库，包括N-丙烯酰-甘氨酸(AGly)、N-丙烯酰-l-苯丙氨酸(APhe)和N -丙烯酰-l-谷氨酸(AGlu)，是根据MC-LR的结构和理化性质分析而选择的。从聚合物库中筛选出对 MC-LR 具有高特异性亲和力的水凝胶共聚物AGlu-NP13（58% AGlu、40% TBAm 和 2% BIS）。氢键相互作用可能主导AGlu-NP13- MC-LR 结合。影响氢键相互作用的外部条件，包括 pH 和溶剂化环境，控制 MC-LR 在AGlu-NP13上的捕获和释放。在 pH 3 的饱和乙酸乙酯（10%，v/v）溶液中，MC-LR 的吸收达到最大。使用 pH 9.5 和 70% 甲醇水溶液，结合的 MC-LR 可以从水凝胶共聚物中完全解吸。筛选出的聚合物亲和配体成功应用于铜绿微囊藻905冻干细胞提取物中MC-LR的分离纯化，为该材料在标准品制备、样品前处理、MC-LR吸附去除等方面提供了潜在的应用前景。复杂的环境和生物样本。