Sensitivity analysis and point mutations in BcSDHB confer cyclobutrifluram resistance in Botrytis cinerea from China,Pesticide Biochemistry and Physiology

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Sensitivity analysis and point mutations in BcSDHB confer cyclobutrifluram resistance in Botrytis cinerea from China
Pesticide Biochemistry and Physiology ( IF 4.7 ) Pub Date : 2024-03-20 , DOI: 10.1016/j.pestbp.2024.105884
Qin Peng , Lijun Tang , Chuang Zhao , Shuailin Liao , Jianqiang Miao , Xili Liu

is one of the most destructive pathogens worldwide. It can damage over 200 crops, resulting in significant yield and quality losses. Cyclobutrifluram, a new generation of succinate dehydrogenase inhibitors, exhibits excellent inhibitory activity against . However, the baseline sensitivity and resistance of to cyclobutrifluram remains poorly understood. This study was designed to monitor the sensitivity frequency distribution, assess the resistance risk, and clarify the resistance mechanism of to cyclobutrifluram. The baseline sensitivity of isolates to cyclobutrifluram was 0.89 μg/mL. Cyclobutrifluram-resistant populations are present in the field. Six resistant isolates investigated in this study possessed enhanced compound fitness index compared to the sensitive isolates according to mycelial growth, mycelial dry weight, conidiation, conidial germination rate, and pathogenicity. Cyclobutrifluram exhibited no cross-resistance with tebuconazole, fludioxonil, cyprodinil, or iprodione. Sequence alignment revealed that BcSDHB from cyclobutrifluram-resistant isolates had three single substitutions (P225F, N230I, or H272R). Molecular docking verified that these mutations in BcSDHB conferred cyclobutrifluram resistance in . In conclusion, the resistance risk of to cyclobutrifluram is high, and the point mutations in BcSDHB (P225F, N230I, or H272R) confer cyclobutrifluram resistance in . This study provided important insights into cyclobutrifluram resistance in and offered valuable information for monitoring and managing cyclobutrifluram resistance in the future.

更新日期：2024-03-20

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