Methamphetamine (MA) is a highly addictive mental stimulant, and MA abuse remains a significant public health problem worldwide, while effective treatment options are limited. Lycium barbarum polysaccharide (LBP), a major effective component extracted from Lycium barbarum, has potential health-promoting effects on the nervous system; however, its role in MA dependence remains unclear. In this study, the conditioned place preference (CPP) of MA addiction in adult male mice was established to detect changes in gut microbiota profiles after LBP treatment through 16S rRNA gene sequencing. Our results found that LBP administration could alleviate MA-induced CPP and hyperactivity. Interestingly, LBP improved MA-induced gut microbiota dysbiosis by increasing some beneficial autochthonous genus abundances, such as Allobaculum, Gordonibacter, and Ileibacterium. MA exposure induced the co-occurrence network of intestinal microbiota to become weaker and more unstable when compared with the control group, while LBP changed the above effects when compared with the MA group. Bacterial gene function prediction showed that amphetamine addiction, cocaine addiction, and short-chain fatty acid metabolism were enriched. These findings reveal that LBP might regulate MA-induced gut microbiota and behavior changes, which showed potential therapeutic applicability in treating MA addiction by regulating the gut microbiota.

中文翻译：

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枸杞多糖减轻甲基苯丙胺成瘾并改变甲基苯丙胺诱导的肠道微生物群失调

甲基苯丙胺 (MA) 是一种高度成瘾的精神兴奋剂，甲基苯丙胺滥用仍然是世界范围内的一个重大公共卫生问题，而有效的治疗选择却很有限。枸杞多糖（LBP）是从枸杞中提取的主要有效成分，对神经系统具有潜在的促进健康作用；然而，其在 MA 依赖中的作用仍不清楚。在这项研究中，建立了成年雄性小鼠MA成瘾的条件性位置偏好（CPP），通过16S rRNA基因测序来检测LBP治疗后肠道微生物群的变化。我们的结果发现 LBP 给药可以减轻 MA 诱导的 CPP 和多动症。有趣的是，LBP 通过增加一些有益的本土菌属丰度（例如异杆菌属、戈登杆菌属和回肠杆菌属）来改善 MA 诱导的肠道微生物群失调。与对照组相比，MA暴露导致肠道菌群共生网络变得更弱且更不稳定，而与MA组相比，LBP改变了上述效应。细菌基因功能预测显示安非他明成瘾、可卡因成瘾和短链脂肪酸代谢丰富。这些发现表明，LBP 可能调节 MA 诱导的肠道微生物群和行为变化，这表明通过调节肠道微生物群治疗 MA 成瘾具有潜在的治疗适用性。