The precise regulation of nanoenzyme activity is of great significance for application to biosensing analysis. Herein, the peroxidase-like activity of carbon dots was effectively modulated by doping phosphorus, which was successfully employed for sensitive, selective detection of acid phosphatase (ACP). Phosphorus-doped carbon dots (P-CDs) with excellent peroxidase-like activity were synthesized by a one-pot hydrothermal method, and the catalytic activity could be easily modulated by controlling the additional amount of precursor phytic acid. P-CDs could effectively catalyze the oxidation of colorless 3,3’,5,5'-tetramethylbenzidine (TMB) to blue TMB oxidation products in the presence of hydrogen peroxide. While ACP was able to catalyze the hydrolysis of L-ascorbyl-2-phosphate trisodium salt (AAP) to produce ascorbic acid (AA), which inhibited the peroxidase-like activity of P-CDs, by combining P-CDs nanoenzymes and ACP-catalyzed hydrolysis the colorimetric method was established for ACP detection. The absorbance variation showed a good linear relationship with ACP concentration in the range of 0.4–4.0 mU/mL with a limit of detection at 0.12 mU/mL. In addition, the method was successfully applied to detect ACP in human serum samples with recoveries in the range of 98.7–101.6%. The work provides an effective strategy for regulating nanoenzymes activity and a low-cost detection technique for ACP.

中文翻译：

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磷调节碳点的类过氧化物酶活性，用于酸性磷酸酶的比色检测

纳米酶活性的精确调控对于生物传感分析的应用具有重要意义。在此，通过掺杂磷有效地调节碳点的类过氧化物酶活性，成功地将其用于酸性磷酸酶（ACP）的灵敏、选择性检测。通过一锅水热法合成了具有优异的过氧化物酶活性的磷掺杂碳点（P-CD），并且通过控制前体植酸的添加量可以轻松调节催化活性。在过氧化氢存在下，P-CDs 可以有效催化无色 3,3',5,5'-四甲基联苯胺 (TMB) 氧化成蓝色 TMB 氧化产物。而ACP通过将P-CDs纳米酶与ACP结合，能够催化L-抗坏血酸-2-磷酸三钠盐（AAP）水解产生抗坏血酸（AA），从而抑制P-CDs的过氧化物酶样活性。建立了催化水解比色法检测ACP。吸光度变化与 ACP 浓度在 0.4-4.0 mU/mL 范围内呈良好的线性关系，检测限为 0.12 mU/mL。此外，该方法还成功应用于人血清样本中ACP的检测，回收率在98.7%~101.6%之间。该工作为调控纳米酶活性提供了有效策略，并为ACP提供了低成本检测技术。