Purpose

The study of nanoparticles for antibacterial, antifungal, and anticancer action is an important sector in nanoscience. The use of plant extracts in nanoparticle synthesis is among the most simple, cost-effective, and ecologically acceptable strategies. The goal is to use Artocarpus altilis fruit extract to generate plant-mediated silver nanoparticles for its biological activity.

Methods

Phytochemical profiling of methanolic extract of Artocarpus altilis fruit was carried out. UV visible spectroscopy and scanning electron microscopy (SEM) are used to characterize the sample. The disc diffusion method was used to interpret antimicrobial activity. The MTT assay was used to determine the cytotoxicity.

Results

The presence of flavonoids, terpenoids, phenolic compounds, and others was discovered by phytochemical profiling. UV-visible spectroscopy was used to establish the existence of AgNPs, and a maximum absorbance range was obtained. The optimized nanoparticles were shown to have a spherical shape by the SEM pictures. Using the disc diffusion method, the defined AgNPs demonstrated antibacterial efficacy against Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis, and Staphylococcus aureus at various doses, as well as antifungal efficacy against Aspergillus fumigates. When compared to doxorubicin, the lung cancer cell lines (A549 cell line) demonstrated potential anticancer activity of silver nanoparticles.

Conclusion

We discovered that silver nanoparticles made from Artocarpus altilis fruit extract have a similar effect to ciprofloxacin. The cytotoxic activity anticipated against the lung cancer cell line (A549 cell line) shows that the percentage of cell viability is identical to that of doxorubicin which indicates the potential for the treatment of lung tumors.

中文翻译：

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菠萝蜜果实提取物生物合成纳米银粒子及其生物活性

目的

纳米粒子的抗菌、抗真菌和抗癌作用的研究是纳米科学的一个重要领域。在纳米颗粒合成中使用植物提取物是最简单、最具成本效益且生态上可接受的策略之一。目标是利用Artocarpus altilis果实提取物生成植物介导的银纳米粒子，以发挥其生物活性。

方法

对Artocarpus altilis果实的甲醇提取物进行了植物化学分析。紫外可见光谱和扫描电子显微镜 (SEM) 用于表征样品。使用纸片扩散法来解释抗菌活性。 MTT法用于测定细胞毒性。

结果

通过植物化学分析发现黄酮类化合物、萜类化合物、酚类化合物和其他化合物的存在。使用紫外-可见光谱来确定AgNPs的存在，并获得最大吸光度范围。 SEM 照片显示优化后的纳米粒子具有球形形状。使用纸片扩散法，确定的AgNPs在不同剂量下表现出对大肠杆菌、铜绿假单胞菌、枯草芽孢杆菌和金黄色葡萄球菌的抗菌功效，以及对烟曲霉的抗真菌功效。与阿霉素相比，肺癌细胞系（A549 细胞系）显示出银纳米颗粒的潜在抗癌活性。

结论

我们发现由菠萝蜜果实提取物制成的银纳米粒子具有与环丙沙星相似的效果。预期针对肺癌细胞系（A549 细胞系）的细胞毒活性表明细胞活力百分比与阿霉素相同，这表明其治疗肺部肿瘤的潜力。