Elucidating the intricate interactions between viral pathogens and host cellular machinery during infection is paramount for understanding pathogenic mechanisms and identifying potential therapeutic targets. The RNA modification N6-methyladenosine (m6A) has emerged as a significant factor influencing the trajectory of viral infections. Hence, the precise and quantitative mapping of m6A modifications in both host and viral RNA is pivotal to understanding its role during viral infection. With the rapid advancement of sequencing technologies, scientists are able to detect m6A modifications with various quantitative, high-resolution, transcriptome approaches. These technological strides have reignited research interest in m6A, underscoring its significance and prompting a deeper investigation into its dynamics during viral infections. This review provides a comprehensive overview of the historical evolution of m6A epitranscriptome sequencing technologies, highlights the latest developments in transcriptome-wide m6A mapping, and emphasizes the innovative technologies for detecting m6A modification. We further discuss the implications of these technologies for future research into the role of m6A in viral infections.

中文翻译：

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解码病毒感染的表观转录调控：通过先进测序技术绘制 RNA N6-甲基腺苷图谱

阐明感染过程中病毒病原体和宿主细胞机制之间复杂的相互作用对于理解致病机制和确定潜在的治疗靶点至关重要。 RNA 修饰 N6-甲基腺苷 (m6A) 已成为影响病毒感染轨迹的重要因素。因此，宿主和病毒 RNA 中 m6A 修饰的精确定量图谱对于理解其在病毒感染过程中的作用至关重要。随着测序技术的快速发展，科学家们能够通过各种定量、高分辨率的转录组方法来检测 m6A 修饰。这些技术进步重新激发了人们对 m6A 的研究兴趣，强调了它的重要性，并促使人们对其在病毒感染过程中的动态进行更深入的研究。本综述全面概述了 m6A 表观转录组测序技术的历史演变，重点介绍了全转录组 m6A 作图的最新进展，并强调了检测 m6A 修饰的创新技术。我们进一步讨论这些技术对 m6A 在病毒感染中的作用的未来研究的影响。