Reactive oxygen species (ROS) are closely associated with the redox balance of the physiological environment, and monitoring ROS can aid in the early diagnosis of many diseases, including cancer. In this study, chiral vanadium trioxide/vanadium nitride (V₂O₃/VN) nanoparticles (NPs) modified with an organic dye (cyanine 3 [Cy3]) were prepared for ROS sensing. Chiral V₂O₃/VN NPs were prepared with the “ligand-induced chirality” strategy and showed a g-factor of up to 0.12 at a wavelength of 512 nm. To the best of our knowledge, this g-factor is the highest value of all chiral ceramic nanomaterials. The very high g-factor of the nanoprobe confers very high sensitivity, because the higher g-factor, the higher sensitivity. In the presence of ROS, V³⁺ in the chiral V₂O₃/VN nanoprobe undergoes a redox reaction to form V₂O₅, reducing the circular dichroism and absorbance signals, whereas the fluorescence signal of Cy3 is restored. With this nanoprobe, the limits of detection for the circular dichroic and fluorescence signals in living cells are 0.0045 nmol/10⁶ and 0.018 nmol/10⁶ cells, respectively. This chiral nanoprobe can also monitor ROS levels in vivo by fluorescence. This strategy provides an innovative approach to the detection of ROS and is expected to promote the wider application of chiral nanomaterials for biosensing.

中文翻译：

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手性钒基纳米颗粒对体内活性氧的三模式响应

活性氧（ROS）与生理环境的氧化还原平衡密切相关，监测ROS可以帮助包括癌症在内的许多疾病的早期诊断。在本研究中，制备了用有机染料（花青3 [Cy3]）修饰的手性三氧化二钒/氮化钒（V ₂ O ₃ /VN）纳米粒子（NP）用于ROS传感。采用“配体诱导手性”策略制备手性 V ₂ O ₃ /VN NP，在 512 nm 波长下显示出高达 0.12 的g因子。据我们所知，该g因子是所有手性陶瓷纳米材料中最高的值。纳米探针的非常高的g因子赋予非常高的灵敏度，因为g因子越高，灵敏度越高。在ROS存在下，手性V ₂ O _{3 /VN纳米探针中的V} ³⁺发生氧化还原反应形成V ₂ O ₅，圆二色性和吸光度信号降低，而Cy3的荧光信号恢复。使用该纳米探针，活细胞中圆二色性和荧光信号的检测限分别为0.0045 nmol/10 ⁶和0.018 nmol/10 ⁶细胞。这种手性纳米探针还可以通过荧光监测体内ROS水平。该策略为ROS的检测提供了一种创新方法，有望促进手性纳米材料在生物传感领域的更广泛应用。