Proteomics of human saliva samples was achieved for the first time via biocompatible solid-phase microextraction (bio-SPME) devices. Upon introduction of a porogen to a conventional C18 coating, porous C18/polyacrylonitrile (PAN) SPME blades were able to extract peptides up to 3.0 kDa and more peptides than commercial SPME blades. Following Trypsin digestion, salivary proteomic analysis was achieved via SPME-LC-MS/MS. Seven endogenous proteins were consistently identified in all saliva samples via bio-SPME. Taking advantage of this strategy, untargeted peptidomics was applied for the comparison of saliva samples between healthy and SARS-CoV-2 positive individuals. The results showed clear peptidomic differences between the viral and healthy saliva samples. This proof-of-concept study demonstrates the potential of bio-SPME-LC-MS/MS for peptidomics and proteomics in biomedical applications.

中文翻译：

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通过固相微萃取结合液相色谱-质谱法对人类唾液进行蛋白质组分析

人类唾液样本的蛋白质组学首次通过生物相容性固相微萃取 (bio-SPME) 装置实现。将致孔剂引入传统 C18 涂层后，多孔 C18/聚丙烯腈 (PAN) SPME 刀片能够提取高达 3.0 kDa 的肽，并且比商用 SPME 刀片提取更多的肽。胰蛋白酶消化后，通过 SPME-LC-MS/MS 实现唾液蛋白质组分析。通过生物固相微萃取，在所有唾液样本中一致鉴定出七种内源蛋白。利用这一策略，应用非靶向肽组学来比较健康个体和 SARS-CoV-2 阳性个体之间的唾液样本。结果显示病毒和健康唾液样本之间存在明显的肽组差异。这项概念验证研究证明了生物固相微萃取-LC-MS/MS 在生物医学应用中肽组学和蛋白质组学的潜力。