Surface enhanced Raman spectroscopy (SERS) is a unique analytical technique with excellent performance in terms of sensitivity, non-destructive detection and resolution. However, due to the randomness and poor repeatability of hot spot distribution, SERS quantitative analysis is still challenging. Meanwhile, snus is a type of tobacco product that can release nicotine and other components in the mouth without burning, and the rapid detection technique based on SERS can reliably evaluate the amount of nicotine released from snus, which is of great significance for understanding its characteristics and regulating its components. Herein, the strategy was proposed to solve the feasibility of SERS quantitative detection based on self-assembled core-shell nanoparticles with embedded internal standards (EIS) due to EIS signal can effectively correct SERS signal fluctuations caused by different aggregation states and measurement conditions, thus allowing reliable quantitative SERS analysis of targets with different surface affinity. By means of process control, after the Au nanoparticles (Au NPs) were modified with 4-Mercaptobenzonitrile (4-MBN) as internal standard molecules, Ag shell with a certain thickness was grown on the surface of the AuNP@4-MBN, and then the Au@4-MBN@Ag NPs were used to regulate and control the assembly of liquid-liquid interface. The high-density nano-arrays assembled at the liquid-liquid interface ensure high reproducibility as SERS substrates, and which could be used for SERS detection of nicotine released from snus products. In addition, time-mapping research shows that this method can also be used to dynamically monitor the release of nicotine. Moreover, such destruction-free evaluation of the release of nicotine from snus products opens up new perspectives for further research about the impact of nicotinoids-related health programs.

中文翻译：

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嵌入内标的自组装核壳纳米粒子，用于 SERS 定量检测和鉴定鼻烟产品中释放的尼古丁

表面增强拉曼光谱（SERS）是一种独特的分析技术，在灵敏度、无损检测和分辨率方面具有优异的性能。然而，由于热点分布的随机性和重复性差，SERS定量分析仍然具有挑战性。同时，口含烟是一种无需燃烧即可在口腔中释放尼古丁等成分的烟草制品，基于SERS的快速检测技术可以可靠地评估口含烟释放的尼古丁量，这对于了解其特性具有重要意义。并调节其成分。由于EIS信号可以有效校正不同聚集状态和测量条件引起的SERS信号波动，因此提出了解决基于嵌入内标（EIS）的自组装核壳纳米颗粒SERS定量检测可行性的策略，从而允许对具有不同表面亲和力的目标进行可靠的定量 SERS 分析。通过工艺控制，以4-巯基苯甲腈（4-MBN）为内标分子对金纳米粒子（Au NPs）进行修饰后，在AuNP@4-MBN表面生长出一定厚度的Ag壳层，然后利用Au@4-MBN@Ag NPs来调控液-液界面的组装。在液-液界面组装的高密度纳米阵列确保了作为SERS基底的高再现性，并且可用于SERS检测鼻烟产品中释放的尼古丁。此外，时间映射研究表明，该方法还可用于动态监测尼古丁的释放。此外，这种对鼻烟产品中尼古丁释放的无破坏评估为进一步研究尼古丁相关健康计划的影响开辟了新的视角。