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Genome-wide analysis of the PG gene family in Penicillium expansum: Expression during the infection stage in pear fruits (Pyrus bretschneideri)
Physiological and Molecular Plant Pathology ( IF 2.7 ) Pub Date : 2024-03-19 , DOI: 10.1016/j.pmpp.2024.102270
Michael Ackah , Nana Adwoa Serwah Boateng , Joice Meshi Foku , Guillaume Legrand Ngolong Ngea , Esa Abiso Godana , Hongyin Zhang , Qiya Yang

is a necrotrophic plant pathogen that has a broad range of fruit hosts and is responsible for blue mold rot. This rot has a significant impact on the fruit industry, causing economic losses during storage, transport, and sale. Polygalacturonase (PGs) enzymes are essential for pathogenic fungi to break down pectin, as they hydrolyze the polygalacturonic acid chain along the oxygen bridge. This enzyme has been identified in decayed tissue and is linked to multiple soft rot diseases. This study encompasses a thorough examination of the gene family within the genome of , utilizing a genome-wide analysis approach. As a result, a total of seven genes were successfully found. These genes were phylogenetically divided into four distinct clades. The gene structural characteristics of each group exhibited a fundamental level of conservation. Furthermore, the gene architectures and motif compositions exhibited robust evidence in favor of the credibility of the phylogenetic relationship. Motif analysis reveals that most of the motifs predicted had Glycosyl hydrolases family 28 conserved domains (PF00295). Analysis of RNA-seq data and the genes identified by RT-qPCR were mostly upregulated. Hence, comprehending the characteristics of holds significant scientific importance and will offer comprehensive insights into the superfamily in and will make a valuable contribution to the ongoing validation of functional genes.

中文翻译:

扩展青霉 PG 基因家族的全基因组分析:在梨果实 (Pyrus bretschneideri) 感染阶段的表达

是一种坏死营养型植物病原体,具有广泛的水果寄主,并导致蓝霉病。这种腐烂对水果产业影响重大,造成贮藏、运输、销售过程中的经济损失。多聚半乳糖醛酸酶(PG)对于病原真菌分解果胶至关重要,因为它们沿着氧桥水解多聚半乳糖醛酸链。这种酶已在腐烂的组织中被发现,并与多种软腐病有关。这项研究利用全基因组分析方法对基因组内的基因家族进行了彻底的检查。结果总共成功找到了7个基因。这些基因在系统发育上分为四个不同的分支。每个群体的基因结构特征都表现出基本的保守水平。此外,基因结构和基序组成显示出强有力的证据支持系统发育关系的可信度。基序分析表明,大多数预测的基序都具有糖基水解酶家族 28 保守结构域 (PF00295)。 RNA-seq数据分析和RT-qPCR鉴定的基因大多上调。因此,了解 的特征具有重要的科学重要性,并将提供对超家族的全面见解,并将为功能基因的持续验证做出宝贵的贡献。
更新日期:2024-03-19
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