Areca concinna Thwaites (Arecaceae) is an endangered plant species endemic to South-Western Sri Lanka and distributed primarily in Sri Lanka, India, and Southeast Asia. This palm was listed under endangered plants species by IUCN (The International Union for Conservation of Nature) due to habitat loss. Clonal propagation is essential for conservation and maintenance of this endangered species. In an effort to conserve this plant species, a protocol was developed for clonal multiplication of A. concinna Thwaites. Immature inflorescence, immature embryos, and mature embryos were tested for callogenesis and subsequent somatic embryogenesis in M72 basal medium supplemented with the auxins 2,4-D and picloram. Of the three explants, somatic embryos were obtained only from mature embryos. Callus multiplication and somatic embryo formation in M72 basal medium supplemented with 2,4-D were found to be better over picloram media. Serial transfer of explants from higher to lower concentrations was essential for sustaining the multiplication of callus as well as for induction of somatic embryos. Somatic embryos grown in Y3 or 1/2 Y3 basal medium supplemented with 1.0 mg L⁻¹ BAP, 0.5 mg L⁻¹ NAA, and 0.25 mg L⁻¹ IBA exhibited shoot initiation and plantlet development. This protocol has its application in rapid multiplication of A. concinna Thwaites palms thereby enhancing the possibility of conservation of the endangered palm.

槟榔科（ Areca concinna Thwaites）是斯里兰卡西南部特有的濒危植物，主要​​分布在斯里兰卡、印度和东南亚。由于栖息地丧失，这种棕榈树被 IUCN（国际自然保护联盟）列为濒危植物物种。克隆繁殖对于保护和维持这种濒临灭绝的物种至关重要。为了保护该植物物种，制定了A. concinna Thwaites 克隆繁殖方案。在补充有生长素2,4-D和毒莠定的M72基础培养基中测试未成熟花序、未成熟胚和成熟胚的愈伤组织和随后的体细胞胚胎发生。在三个外植体中，体细胞胚胎仅从成熟胚胎中获得。发现补充有 2,4-D 的 M72 基础培养基中的愈伤组织增殖和体细胞胚胎形成优于毒莠定培养基。外植体从较高浓度到较低浓度的连续转移对于维持愈伤组织的增殖以及体细胞胚胎的诱导至关重要。在补充有1.0 mg L ^-1 BAP、0.5 mg L ^-1 NAA和0.25 mg L ^-1 IBA的Y3或1/2 Y3基础培养基中生长的体细胞胚表现出芽起始和幼苗发育。该协议应用于A. concinna Thwaites 棕榈的快速繁殖，从而提高了濒危棕榈保护的可能性。