Rapidly evolving bacterial pathogens pose a unique challenge for long-term plant disease management. In this study, we investigated the types and rate of mutations in bacterial populations during seasonal disease epidemics. Two phylogenetically distinct strains of the bacterial spot pathogen, Xanthomonas perforans, were marked, released in tomato fields, and recaptured at several time points during the growing season. Genomic variations in recaptured isolates were identified by comparative analysis of their whole-genome sequences. In total, 180 unique variations (116 substitutions, 57 insertions/deletions, and 7 structural variations) were identified from 300 genomes, resulting in the overall host-associated mutation rate of ∼0.3 to 0.9/genome/week. This result serves as a benchmark for bacterial mutation during epidemics in similar pathosystems.

Copyright © 2024 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.

快速进化的细菌病原体对长期植物病害管理提出了独特的挑战。在这项研究中，我们研究了季节性疾病流行期间细菌种群突变的类型和比率。两种系统发育不同的细菌斑病病原体菌株——穿孔黄单胞菌（Xanthomonas perforans）被标记出来，释放到番茄田中，并在生长季节的几个时间点重新捕获。通过对全基因组序列进行比较分析，鉴定了重新捕获的分离株的基因组变异。总共，从 300 个基因组中鉴定出 180 个独特变异（116 个替换、57 个插入/缺失和 7 个结构变异），导致总体宿主相关突变率为 ∼0.3 至 0.9/基因组/周。这一结果可作为类似病理系统流行期间细菌突变的基准。

版权所有 © 2024 作者。这是一篇根据 CC BY-NC-ND 4.0 国际许可证分发的开放获取文章。