Abstract

Recently, numerous studies including various tissues have been carried out on long non-coding RNAs (lncRNAs), but still, its variability has not yet been fully understood. In this study, we characterised the inter-individual variability of lncRNAs in pigs, in the context of number, length and expression. Transcriptomes collected from muscle tissue belonging to six Polish Landrace boars (PL1–PL6), including half-brothers (PL1–PL3), were investigated using bioinformatics (lncRNA identification and functional analysis) and statistical (lncRNA variability) methods. The number of lncRNA ranged from 1289 to 3500 per animal, and the total number of common lncRNAs among all boars was 232. The number, length and expression of lncRNAs significantly varied between individuals, and no consistent pattern has been found between pairs of half-brothers. In detail, PL5 exhibits lower expression than the others, while PL4 has significantly higher expression than PL2–PL3 and PL5–PL6. Noteworthy, comparing the inter-individual variability of lncRNA and mRNA expression, they exhibited concordant patterns. The enrichment analysis for common lncRNA target genes determined a variety of biological processes that play fundamental roles in cell biology, and they were mostly related to whole-body homeostasis maintenance, energy and protein synthesis as well as dynamics of multiple nucleoprotein complexes. The high variability of lncRNA landscape in the porcine genome has been revealed in this study. The inter-individual differences have been found in the context of three aspects: the number, length and expression of lncRNAs, which contribute to a better understanding of its complex nature.

中文翻译：

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猪骨骼肌中长非编码RNA变异

摘要

最近，人们对长链非编码RNA（lncRNA）进行了包括各种组织在内的大量研究，但其变异性尚未完全了解。在这项研究中，我们从数量、长度和表达的角度描述了猪 lncRNA 的个体差异。使用生物信息学（lncRNA 鉴定和功能分析）和统计（lncRNA 变异性）方法对从六头波兰长白公猪（PL1-PL6）（包括同父异母兄弟（PL1-PL3））的肌肉组织中收集的转录组进行了研究。每只公猪的lncRNA数量范围为1289至3500个，所有公猪中常见lncRNA的总数为232个。lncRNA的数量、长度和表达在个体之间显着差异，并且在成对的半公猪之间没有发现一致的模式。兄弟。具体而言，PL5 的表达量低于其他细胞，而 PL4 的表达量显着高于 PL2-PL3 和 PL5-PL6。值得注意的是，比较 lncRNA 和 mRNA 表达的个体间差异，它们表现出一致的模式。对常见lncRNA靶基因的富集分析确定了在细胞生物学中发挥基础作用的多种生物过程，它们大多与全身稳态维持、能量和蛋白质合成以及多种核蛋白复合物的动力学相关。这项研究揭示了猪基因组中 lncRNA 景观的高度变异性。在lncRNA的数量、长度和表达三个方面发现了个体间差异，这有助于更好地理解其复杂性质。