Maintaining the continuous oxygen supply and proper cell growth before blood vessel ingrowth at the bone defect site are considerably significant issues in bone regeneration. Oxygen-producing scaffolds can supply oxygen and avoid hypoxia leading to expedited bone regeneration. Herein, first oxygen-producing calcium peroxide nanoparticles (CPO NPs) are synthesized, and subsequently, the various amounts of synthesized CPO NPs (0.1, 0.5, and 1 wt/v%) loaded in the scaffold composite, which is developed by simple physical blending of chitosan (CS) and polycaprolactone (PCL) polymers. To deliver the synergistic therapeutic effect, dexamethasone (DEX), known for its potential anti-inflammatory and osteogenic properties, is loaded into the nanocomposite scaffolds. The extensive physicochemical characterizations of nanocomposite scaffolds confirm the successful loading of CPO NPs, adequate porous morphology, pore size, hydrophilicity, and biodegradability. In vitro, biological studies support the antibacterial, hemocompatible, and cytocompatible (MG-63 and MC3T3-E1 cells) nature of the material when tested on respective cells. Field emission scanning electron microscopy and energy-dispersive x-ray spectroscopy confirm the successful biomineralization of the scaffolds. Scaffolds also exhibit the sustained release of DEX and efficient protein adsorption. This study revealed that a nanoengineered scaffold loaded with CPO NPs (PCL/CS/DEX/CPO 3) is a suitable candidate for bone tissue regeneration.

中文翻译：

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持续释放地塞米松的纳米工程释氧聚合物支架用于骨再生

在骨缺损部位的血管向内生长之前保持持续的氧气供应和适当的细胞生长是骨再生中相当重要的问题。产氧支架可以提供氧气并避免缺氧，从而加速骨再生。在此，首先合成了产氧的过氧化钙纳米颗粒（CPO NP），然后将不同量的合成 CPO NP（0.1、0.5 和 1 wt/v%）负载到支架复合材料中，该支架复合材料是通过简单的物理方法开发的。壳聚糖 (CS) 和聚己内酯 (PCL) 聚合物的共混物。为了提供协同治疗效果，将以其潜在的抗炎和成骨特性而闻名的地塞米松（DEX）加载到纳米复合材料支架中。纳米复合材料支架的广泛物理化学表征证实了 CPO NP 的成功负载、足够的多孔形态、孔径、亲水性和生物降解性。体外，在各自的细胞上进行测试时，生物学研究支持材料的抗菌、血液相容性和细胞相容性（MG-63 和 MC3T3-E1 细胞）性质。场发射扫描电子显微镜和能量色散 X 射线光谱证实了支架的成功生物矿化。支架还表现出 DEX 的持续释放和高效的蛋白质吸附。这项研究表明，载有 CPO NP (PCL/CS/DEX/CPO 3) 的纳米工程支架是骨组织再生的合适候选者。