Corneal stromal injury is a common surgical disease. With the development of tissue engineering materials, many artificial corneal scaffolds have been developed to replace allograft corneal transplantation and solve the problem of corneal donor shortage. However, few researchers have paid attention to corneal stromal wound healing. Herein, a nanocomposite of amino modified mesoporous bioactive glass (MBG-NH₂) and microRNA-133b (miR-133b) was introduced into the patterned collagen films to achieve corneal stromal injury repair. MBG-NH₂ nanoparticles as a nano delivery carrier could efficiently load miR-133b and achieve the slow release of miR-133b. The physicochemical properties of collagen films were characterized and found the microgrooved collagen films loaded with miR-133b@MBG-NH₂ nanoparticles possessed similar swelling properties, optical clarity, and biodegradability to the natural cornea. In vitro cell experiments were also conducted and proved that the patterned collagen films with miR-133b@MBG-NH₂ possessed good biocompatibility, and miR-133b@MBG-NH₂ nanoparticles could be significantly uptake by rabbit corneal stromal cells (RCSCs) and have a significant impact on the orientation, proliferation, migration, and gene expression of RCSCs. More importantly, the patterned collagen films with miR-133b@MBG-NH₂ could effectively promote the migration of RCSCs and accelerate wound healing process, and down-regulate the expression levels of α-SMA, COL-I, and CTGF genes associated with myofibroblast differentiation of corneal stromal cells, which has a potential application prospect in the repair of corneal stromal injury.

中文翻译：

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载有 miR-133b@MBG-NH2 的图案化胶原蛋白膜在角膜基质损伤修复中的潜在应用

角膜基质损伤是一种常见的外科疾病。随着组织工程材料的发展，许多人工角膜支架被研制出来，以替代同种异体角膜移植，解决角膜供体短缺的问题。然而，很少有研究人员关注角膜基质伤口愈合。在此，将氨基改性介孔生物活性玻璃（MBG-NH ₂）和微小RNA-133b（miR-133b）的纳米复合材料引入图案化胶原膜中以实现角膜基质损伤修复。 MBG-NH ₂纳米颗粒作为纳米递送载体可以有效负载miR-133b并实现miR-133b的缓慢释放。对胶原膜的理化性质进行了表征，发现负载miR-133b@MBG-NH ₂纳米粒子的微槽胶原膜具有与天然角膜相似的溶胀特性、光学透明度和生物降解性。体外细胞实验证明，miR-133b@MBG-NH ₂图案化胶原膜具有良好的生物相容性，miR-133b@MBG-NH ₂纳米颗粒可被兔角膜基质细胞（RCSCs）显着摄取，并具有良好的生物相容性。对 RCSC 的定向、增殖、迁移和基因表达产生显着影响。更重要的是，带有miR-133b@MBG-NH ₂的图案化胶原膜可以有效促进RCSCs的迁移并加速伤口愈合过程，并下调RCSCs的表达水平α-SMA、COL-I、CTGF基因与角膜基质细胞肌成纤维细胞分化相关，在角膜基质损伤修复中具有潜在的应用前景。