The present work focuses on developing 5% w/v oxidized alginate (alginate di aldehyde, ADA)-7.5% w/v gelatin (GEL) hydrogels incorporating 0.25% w/v silk fibroin (SF) and loaded with 0.3% w/v Cu-Ag doped mesoporous bioactive glass nanoparticles (Cu-Ag MBGNs). The microstructural, mechanical, and biological properties of the composite hydrogels were characterized in detail. The porous microstructure of the developed ADA-GEL based hydrogels was confirmed by scanning electron microscopy, while the presence of Cu-Ag MBGNs in the synthesized hydrogels was determined using energy dispersive x-ray spectroscopy. The incorporation of 0.3% w/v Cu-Ag MBGNs reduced the mechanical properties of the synthesized hydrogels, as investigated using micro-tensile testing. The synthesized ADA-GEL loaded with 0.25% w/v SF and 0.3% w/v Cu-Ag MBGNs showed a potent antibacterial effect against Escherichia coli and Staphylococcus aureus. Cellular studies using the NIH3T3-E1 fibroblast cell line confirmed that ADA-GEL films incorporated with 0.3% w/v Cu-Ag MBGNs exhibited promising cellular viability as compared to pure ADA-GEL (determined by WST-8 assay). The addition of SF improved the biocompatibility, degradation rate, moisturizing effects, and stretchability of the developed hydrogels, as determined in vitro. Such multimaterial hydrogels can stimulate angiogenesis and exhibit desirable antibacterial properties. Therefore further (in vivo) tests are justified to assess the hydrogels’ potential for wound dressing and skin tissue healing applications.

中文翻译：

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氧化藻酸盐明胶 (ADA-GEL)/丝素蛋白/Cu-Ag 掺杂介孔生物活性玻璃纳米颗粒水凝胶，用于潜在的伤口护理治疗

目前的工作重点是开发 5% w/v 氧化海藻酸盐（海藻酸二醛，ADA）-7.5% w/v 明胶 (GEL) 水凝胶，其中掺入 0.25% w/v 丝素蛋白 (SF) 并负载 0.3% w/v Cu-Ag 掺杂介孔生物活性玻璃纳米颗粒（Cu-Ag MBGN）。详细表征了复合水凝胶的微观结构、机械和生物特性。通过扫描电子显微镜证实了所开发的 ADA-GEL 水凝胶的多孔微观结构，同时使用能量色散 X 射线光谱法测定了合成水凝胶中 Cu-Ag MBGN 的存在。根据微拉伸测试的研究，加入 0.3% w/v Cu-Ag MBGN 降低了合成水凝胶的机械性能。合成的 ADA-GEL 负载 0.25% w/v SF 和 0.3% w/v Cu-Ag MBGN，对细菌表现出有效的抗菌作用。大肠杆菌和金黄色葡萄球菌。使用 NIH3T3-E1 成纤维细胞系进行的细胞研究证实，与纯 ADA-GEL（通过 WST-8 测定法测定）相比，掺有 0.3% w/v Cu-Ag MBGN 的 ADA-GEL 薄膜表现出良好的细胞活力。经测定，SF 的添加提高了所开发水凝胶的生物相容性、降解率、保湿效果和拉伸性体外。这种多材料水凝胶可以刺激血管生成并表现出理想的抗菌特性。因此进一步(体内）测试可以评估水凝胶在伤口敷料和皮肤组织愈合应用中的潜力。