A comprehensive understanding of the factors and ability of cellulase enzyme to completely hydrolyze the structural lignocellulosic biomass has been a major research focus over the years. A comparative analysis of cellulose-cellulase digestibility of Kraft-pretreated sawdust from twenty different Nigeria wood wastes was carried out with increasing enzyme (Trichorderma viride) loading of 0.4 mg cm⁻³, 1.0 mg cm⁻³, 2.0 mg cm⁻³, and 4.0 mg cm⁻³ at constant substrate application of 10 mg cm⁻³ biomass concentration, temperature of 40°C and 2 h incubation period. This was carried out in order to establish the optimum cellulase-cellulose ratio for maximum biodegradation of the lignocellulosic biomass to produce glucose, a fermentable sugar. The influence of the cellulose-cellulase interaction from 0.4 mg cm⁻³ to 1.0 mg cm⁻³ enzyme treatment resulted in 144% increase in glucose yield from E. suaveolen and 121% from S. pustlatas. while 2.0 mg cm⁻³ cellulase concentration gave 674%, 641% and 617% increase from E.suaveolen, H. ciliate and A. germinans respectively. A general trend of increasing sugar formation was observed with an increasing enzyme loading due to enhanced cellulose accessibility by the cellulase enzyme leading to effective saccharification of the lignocellulosic materials for more sugar production. However, further increase of 4.0 mg cm⁻³ enzyme concentration failed to produce a commiserate amount of fermentable sugar.

中文翻译：

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纤维素-纤维素酶消化率对木质纤维素生物质废弃物生产葡萄糖的影响

全面了解纤维素酶完全水解结构木质纤维素生物质的因素和能力一直是多年来的主要研究热点。随着酶的增加，对来自二十种不同尼日利亚木材废料的硫酸盐预处理锯末的纤维素-纤维素酶消化率进行了比较分析（绿色木霉^{）在10 mg cm -3}生物量浓度的恒定底物应用下，加载0.4 mg cm ^-3、1.0 mg cm ^-3、2.0 mg cm ^-3和4.0 mg cm ^-3，温度为40°C，孵育时间为2 h 。这样做是为了建立最佳的纤维素酶-纤维素比例，以最大程度地生物降解木质纤维素生物质以产生葡萄糖（一种可发酵的糖）。纤维素-纤维素酶相互作用的影响从0.4 mg cm ^-3到1.0 mg cm ^-3酶处理导致葡萄糖产量增加144%E. suaveolen121% 来自S. pustlatas。而2.0 mg cm ^-3纤维素酶浓度则分别增加了674%、641%和617%E.suaveolen, H. 纤毛虫和日发放线菌分别。随着酶负载的增加，观察到糖形成增加的总体趋势，这是由于纤维素酶对纤维素的可及性增强，导致木质纤维素材料的有效糖化以产生更多的糖。然而，进一步增加4.0mg cm ^-3酶浓度未能产生相应量的可发酵糖。