Abstract

Stemphylium blight (SB) is one of the most important biotic stresses of lentil, occuring in almost all major lentil-growing nations. Among 150 species recorded, Stemphylium botryosum (Wallr.) is responsible for inciting SB in lentil. It has a wide host range and perpetuate in the seeds, crop debris and secondary spread occurs through air-borne conidia. The most vulnerable stage of infection is flowering to early pod setting, so, confers a serious threat to lentil production. Environment plays pivotal role in host–pathogen dynamics and also interfere in disease management. This study involves deciphering genotype × environment interactions by utilising GGE biplot to unveil and validate durable resistant genotypes and their performance over three different locations. Primarily, from 70 lentil genotypes, 23 promising genotypes were selected based on their disease reaction (< 30%) against SB and evaluated further across the three locations for two consecutive years. The test result from GGE biplot analysis revealed that genotype exerted higher proportion of variation in SB reaction than environment. Result confirmed the presence of inconsistent genotypic response over locations and years. Selected locations grouped into three environment clusters suggesting the existence of cross over interaction with in the genotypes. Integration of GGE biplot and BLUP analysis identified G-3 (ILL10838/ILWL11/X2016S) as the “ideal” genotype whereas, G-7 (8114/10956/16–1) and G-5 (LIRL22-107/LIRL21-50–1-1–1) as “desirable” genotypes considering their static response against SB over the environment. Genotypes detected through this study could be recommended for their future deployment in lentil resistance breeding programme.

中文翻译：

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GGE双标图技术描绘基因型×环境相互作用，以确定扁豆-Stemphylium枯萎病病理系统中的稳定抗性来源

摘要

茎叶枯病 (SB) 是扁豆最重要的生物胁迫之一，几乎发生在所有主要扁豆种植国家。在记录的 150 个物种中，Stemphylium botryosum (Wallr.) 负责刺激扁豆中的 SB。它具有广泛的寄主范围，并长期存在于种子、农作物残体中，并通过空气传播的分生孢子进行二次传播。最容易受到感染的阶段是开花到结荚早期，因此对扁豆生产造成严重威胁。环境在宿主-病原体动态中发挥着关键作用，也干扰疾病管理。这项研究涉及通过利用 GGE 双标图来揭示和验证持久抗性基因型及其在三个不同位置的表现，从而破译基因型与环境的相互作用。首先，根据对 SB 的疾病反应（< 30%），从 70 个扁豆基因型中选择了 23 个有前途的基因型，并连续两年在三个地点进行了进一步评估。 GGE双标图分析的测试结果表明，基因型对SB反应的变异比例高于环境。结果证实了不同地点和年份之间存在不一致的基因型反应。选定的位置分为三个环境簇，表明基因型中存在交叉相互作用。 GGE 双图和 BLUP 分析的整合确定 G-3 (ILL10838/ILWL11/X2016S) 为“理想”基因型，而 G-7 (8114/10956/16–1) 和 G-5 (LIRL22-107/LIRL21-50) –1-1-1) 作为“理想”基因型，考虑到它们对环境中 SB 的静态反应。通过这项研究检测到的基因型可以推荐用于未来在扁豆抗性育种计划中的部署。