Hypochlorite plays an important role in life system, so it is necessary to develop an effective method to detect hypochlorite. In view of the advantages of fluorescent probes at present, we constructed two novel fluorescent probes (, based on phenothiazine, which can be used for specific detection of ClO. Due to the introduction of nitrogen-containing heterocyclic quinazolinone, shows better optical properties: a great stokes shift (190 nm), excellent selectivity and anti-interference, low detection limit (0.054 μM) and fast response time (20 s). The color change of ClO identified by the probe can be observed with naked eyes under sunlight. Through the calculation of density functional theory and mass spectrometry experiments, the mechanism of fluorescence detection of ClO by was further verified. Furthermore, also realized the detection of ClO in actual water samples and test strips, and achieved remarkable results. Due to its better optical properties and low cytotoxicity, probe has been successfully applied to the fluorescence detection of ClO in living cells.

中文翻译：

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两种基于吩噻嗪的具有不同荧光信号的新型ClO−特异性检测荧光探针及其应用

次氯酸盐在生命系统中发挥着重要作用，因此有必要开发一种有效的次氯酸盐检测方法。鉴于目前荧光探针的优点，我们构建了两种新型荧光探针（基于吩噻嗪，可用于ClO的特异性检测。由于含氮杂环喹唑啉酮的引入，表现出更好的光学性质：斯托克斯位移大（190 nm），优异的选择性和抗干扰性，检测限低（0.054 μM），响应时间快（20 s），在阳光下肉眼即可观察到ClO的颜色变化。通过密度泛函理论计算和质谱实验，进一步验证了ClO荧光检测的机理，并且还实现了实际水样和试纸中ClO的检测，并由于其较好的光学性质取得了显着的效果。且细胞毒性低，探针已成功应用于活细胞中ClO的荧光检测。