This study was to demonstrate that the alleviate effect of synbiotics ( and xylo-oligosaccharide) on NaHCO-induced intestinal barrier dysfunction and autophagy of crucian carp (). In a 2×2 factorial design, crucian carp were divided into 4 groups, fed with diets contained two levels of synbiotics (0 and 1× 10 CFU/g) and treated with two levels of NaHCO (0 and 50 mmol/L) for 56 days. The results showed that NaHCO and synbiotics significantly affected intestinal fold height, permeability, the mRNA expression of tight junction, antioxidant capacity, immunity function (except LZM), inflammation response, the mRNA and protein expression of autophagy (except Beclin1 protein) and PI3K/Akt pathway (<0.05). Intestinal muscular layer thickness, the levels of LZM and the protein expression of Beclin1 only affected by NaHCO (<0.05). In addition, the interaction between NaHCO and synbiotics was observed for intestinal fold height, the mRNA expression of , , , , , , GPX4b, , , , and , the levels of ROS and ACP, the protein expression of p-PI3K/PI3K, p-Akt/Akt, ATG5, ATG7, Beclin1 and LC3Ⅱ/LC3Ⅰ (<0.05). Additionally, this study highlights that the synbiotics facilitated the colonisation and , while concurrently inhibited the growth of in intestines. These findings suggested that dietary synbiotics ( and xylo-oligosaccharide) can effectively alleviated NaHCO-induced intestinal dysfunction oxidative stress and autophagy by regulating intestinal microflora and PI3K/Akt pathway.

中文翻译：

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枯草芽孢杆菌和低聚木糖通过调节鲫鱼肠道菌群和 PI3K/Akt 通路改善 NaHCO3 诱导的肠道屏障功能障碍和自噬

本研究旨在证明合生元（和低聚木糖）对 NaHCO 诱导的鲫鱼肠道屏障功能障碍和自噬的缓解作用。在2×2析因设计中，将鲫鱼分为4组，饲喂含有两种水平的合生素（0和1×10 CFU/g）的饲料，并用两种水平的NaHCO3（0和50mmol/L）处理。 56天。结果表明，NaHCO3和合生元显着影响肠皱襞高度、通透性、紧密连接mRNA表达、抗氧化能力、免疫功能（除LZM）、炎症反应、自噬（除Beclin1蛋白）和PI3K/的mRNA和蛋白表达。 Akt 途径（<0.05）。肠肌层厚度、LZM水平和Beclin1蛋白表达仅受NaHCO影响(<0.05)。此外，还观察了NaHCO3与合生元之间的相互作用，观察了肠褶皱高度、、、、、、、、、、GPX4b、、、、、和、的mRNA表达、ROS和ACP的水平、p-PI3K/PI3K的蛋白表达、 p-Akt/Akt、ATG5、ATG7、Beclin1 和 LC3Ⅱ/LC3Ⅰ（<0.05）。此外，这项研究强调，合生元促进了 和 的定植，同时抑制了 和 在肠道中的生长。这些研究结果表明，膳食合生元（和低聚木糖）可以通过调节肠道菌群和 PI3K/Akt 通路，有效缓解 NaHCO3 诱导的肠道功能障碍氧化应激和自噬。