Three experimental trials were conducted to investigate the influence of shade and cold stratification on germination success of vegetative propagules from multiple genotypes. Shade level did not significantly impact germination of propagules in trial 1 (p=0.16); however, significant differences (p<0.0001) in germination percentages at the conclusion of the study were detected between genotypes. Rhizome segments of the triploid genotype one (G1) had the highest mean germination (95±1%); whereas bulbils of the diploid genotypes three (G3), four (G4), and five (G5) germinated to 9±2%, 1±1%, and 15±2%, respectively. Trial 2 focused on bulbils from G3, G4, and G5 diploid plants that were stratified at 4℃ for 35d. Like Trial 1, shade level was not significant (p=0.19) relative to the overall germination of cold-stratified bulbils. However, cold-stratified bulbils exhibited a much higher mean germination (≥93%) for all three genotypes. In Trial 3, the cold stratification treatments were significant and positively correlated to overall germination for G4 (p=0.005, r=0.77) and G5 (p=0.002, r=0.82), but not G3 (p=0.22, r=0.40) bulbils. Germination time significantly differed between genotypes in all cold-stratification treatments except for the 0, 120, and 180-day treatments. These studies demonstrate that a high percentage of vegetative propagules produced by are capable of successfully germinating under laboratory conditions, but some require extended periods of cold exposure. Given that a single diploid bulbil can produce thousands of bulbils within a growing season; long term management of this species will need to be focused towards limiting bulbil production.

中文翻译：

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遮荫和冷层积对多种伞形鳅基因型营养繁殖体萌发成功率的影响

进行了三项实验来研究遮荫和冷层积对多种基因型营养繁殖体发芽成功的影响。在试验 1 中，遮荫水平没有显着影响繁殖体的萌发（p=0.16）；然而，在研究结束时发现基因型之间的发芽率存在显着差异（p<0.0001）。三倍体基因型一（G1）的根茎段平均发芽率最高（95±1%）；而二倍体基因型三 (G3)、四 (G4) 和五 (G5) 的珠芽萌发率分别为 9±2%、1±1% 和 15±2%。试验2集中于G3、G4和G5二倍体植物的珠芽，在4℃下层积35天。与试验 1 一样，遮荫水平相对于冷分层珠芽的整体萌发并不显着（p=0.19）。然而，对于所有三种基因型，冷分层珠芽均表现出更高的平均发芽率（≥93%）。在试验 3 中，冷分层处理与 G4（p=0.005，r=0.77）和 G5（p=0.002，r=0.82）的总体发芽率显着呈正相关，但与 G3（p=0.22，r=0.40）无关。 ）珠芽。除 0、120 和 180 天处理外，所有冷分层处理中不同基因型的发芽时间均显着不同。这些研究表明，高比例的无性繁殖体能够在实验室条件下成功发芽，但有些需要长时间的冷暴露。鉴于单个二倍体珠芽可以在生长季节内产生数千个珠芽；该物种的长期管理需要集中于限制珠芽产量。