Nitroreductase (NTR) is overexpressed in hypoxic tumor cells, which is closely related to inflammation, tumorigenesis, tumor invasion and metastasis. Therefore, developing fluorescence probes for detecting intracellular NTR sensitivity has great significance for diagnosis and treatment of tumors. Therefore, we designed a novel fluorescent probe () based on julolidine skeleton for the detection of NTR. The probe could discriminate rapidly NTR from other biological species like sulfhydryl compounds and some ions. Meanwhile, a fine linear relationship between fluorescence intensity of the probe and NTR consistence has been observed with a very low detection limit of 4.4 ng/mL. In addition, was successfully applied in imaging endogenous NTR in Hela cells and living zebrafish. The excellent performance of the probe makes it have great potential for monitoring NTR in complex biological systems, and it also provides the possibility of its application in human health, disease diagnosis and treatment.

中文翻译：

---

开发基于久洛里定的高灵敏度荧光探针，用于检测活细胞和斑马鱼中的硝基还原酶

硝基还原酶（NTR）在缺氧的肿瘤细胞中过度表达，与炎症、肿瘤发生、肿瘤侵袭和转移密切相关。因此，开发检测细胞内NTR敏感性的荧光探针对于肿瘤的诊断和治疗具有重要意义。因此，我们设计了一种基于久洛里定骨架的新型荧光探针（）用于检测NTR。该探针可以快速区分 NTR 与其他生物物种，如巯基化合物和一些离子。同时，探针的荧光强度与 NTR 浓度之间存在良好的线性关系，检测限极低，为 4.4 ng/mL。此外，还成功应用于 Hela 细胞和活体斑马鱼的内源性 NTR 成像。该探针的优异性能使其在监测复杂生物系统中的NTR方面具有巨大潜力，也为其在人类健康、疾病诊断和治疗中的应用提供了可能。