Regioselective enzymatic polycondensation of the bio‐based cellulose derived polyol, Triol‐citro, and dimethyl adipate using Candida antarctica Lipase B (CaLB) was investigated. A Design of Experiment approach with MODDE® Pro 13 was used to determine important factors in the branching behavior of this polymer, and reactant ratio, temperature, reaction time and enzyme wt% were the studied factors. Multifunctional polyesters with pendant hydroxyl groups were synthesized and fully characterized using 2D NMR techniques to determine degree of branching. Branching was minimal, with a maximum of 16% observed, and monomer ratio, temperature and reaction time were all determined to be significant factors. In this work, Mn of up to 13 kDa were achieved, while maintaining degree of branching below 15%, resulting in a linear polyester with the potential to be further functionalized.

中文翻译：

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基于纤维素衍生三醇单体的聚酯的受控酶法合成：实验方法设计

研究了使用南极假丝酵母脂肪酶 B (CaLB) 对生物基纤维素衍生的多元醇、三醇-柠檬酸和己二酸二甲酯进行区域选择性酶促缩聚。采用 MODDE® Pro 13 的实验设计方法来确定该聚合物支化行为的重要因素，反应物比例、温度、反应时间和酶重量百分比是研究因素。合成了带有羟基侧基的多官能聚酯，并使用 2D NMR 技术对其进行了全面表征以确定支化度。支化最少，观察到的最大为 16%，单体比例、温度和反应时间均被确定为重要因素。在这项工作中，Mn 达到了高达 13 kDa，同时保持支化度低于 15%，从而产生了具有进一步功能化潜力的线性聚酯。