Patient-derived organoid models of estrogen receptor-positive (ER+) breast cancer would provide a much-needed tool to understand drug resistance and disease progression better. However, the establishment and long-term maintenance of ER expression, function, and response in vitro remains a significant challenge. Here, we report the development of an ER+ breast tumor organoid medium (BTOM-ER) that conserves ER expression, estrogen responsiveness, and dependence, as well as sensitivity to endocrine therapy of ER+ patient-derived xenograft organoids (PDXO). Our findings demonstrate the utility of subtype-specific culture conditions that better mimic the characteristics of the breast epithelial biology and microenvironment, providing a powerful platform for investigating therapy response and disease progression of ER+ breast cancer.

中文翻译：

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建立雌激素受体阳性乳腺癌类器官模型生成和维持的条件

源自患者的雌激素受体阳性（ER+）乳腺癌类器官模型将为更好地了解耐药性和疾病进展提供急需的工具。然而，体外ER表达、功能和反应的建立和长期维持仍然是一个重大挑战。在此，我们报告了 ER+ 乳腺肿瘤类器官培养基 (BTOM-ER) 的开发，该培养基保留 ER 表达、雌激素反应性和依赖性，以及 ER+ 患者来源的异种移植类器官 (PDXO) 对内分泌治疗的敏感性。我们的研究结果证明了亚型特异性培养条件的实用性，可以更好地模拟乳腺上皮生物学和微环境的特征，为研究 ER+ 乳腺癌的治疗反应和疾病进展提供强大的平台。