In this study, zinc oxide nanoparticles (ZnO-NPs) are synthesized and combined with chitosan (Cs) to create Cs/ZnO-NPs nanomicelles, aiming to investigate their potential as a novel cancer treatment. The ZnO-NPs are produced through a sintering process at temperatures ranging from 300 to 700 °C. The most effective nanoparticles are obtained at 600 °C, as determined by X-ray diffraction (XRD) and Fourier-transform infrared spectroscopy (FTIR) analyses, which confirmed their crystallinity and purity. Transmission electron microscopy (TEM) and scanning electron microscopy (SEM) are employed to characterize the size and shape of the nanoparticles, revealing predominantly spherical and hexagonal structures with stable dimensions. The cytotoxic effects of the Cs/ZnO-NPs are evaluated against various cancer cell types. The results show that at a concentration of 125 µg mL⁻¹, the Cs/ZnO-NPs demonstrate significantly higher cancer cell toxicity compared to ZnO-NPs alone, while remaining non-toxic to normal cells. This indicates that Cs/ZnO-NPs have a superior ability to selectively target cancer cells. These findings suggest that Cs/ZnO-NPs nanomicelles hold promise as an effective and safe nanotherapeutic approach in the realm of cancer treatment, meriting further exploration for clinical applications.

中文翻译：

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ZnO纳米颗粒的绿色化学合成方法及其细胞毒性研究

在这项研究中，合成了氧化锌纳米粒子（ZnO-NPs）并与壳聚糖（Cs）结合形成Cs/ZnO-NPs纳米胶束，旨在研究它们作为新型癌症治疗的潜力。 ZnO-NPs是在300至700°C的温度下通过烧结工艺生产的。最有效的纳米粒子是在 600 °C 下获得的，通过 X 射线衍射 (XRD) 和傅里叶变换红外光谱 (FTIR) 分析确定，这证实了它们的结晶度和纯度。采用透射电子显微镜（TEM）和扫描电子显微镜（SEM）来表征纳米粒子的尺寸和形状，显示出主要为具有稳定尺寸的球形和六边形结构。针对各种癌细胞类型评估了 Cs/ZnO-NP 的细胞毒性作用。结果表明，在125 µg mL ^-1的浓度下，Cs/ZnO-NPs 与单独的 ZnO-NPs 相比表现出显着更高的癌细胞毒性，同时对正常细胞保持无毒性。这表明Cs/ZnO-NPs具有优异的选择性靶向癌细胞的能力。这些发现表明，Cs/ZnO-NPs 纳米胶束有望成为癌症治疗领域一种有效且安全的纳米治疗方法，值得进一步探索临床应用。