This study investigated the fungicidal efficiency and mechanism of action of dielectric barrier discharge cold atmosphere plasma (DBD-CAP) in inactivating () spores. The disinfection efficacy and quality of dried jujube used as the processing application object were also studied. The results indicated that the Weibull + Tail model performed better for spore inactivation curves at different voltages among various treatment times, and the spore cells were reduced by 4.05 log (cfu/mL) in spores suspension at 70 kV after 15 min of treatment. This disinfection impact was further supported by scanning electron microscope (SEM) and transmission electron microscopy (TEM) images, which showed that the integrity of the cell membrane was damaged, and the intracellular content leaked out after DBD-CAP treatment. Elevated levels of reactive oxygen species (ROS) during the treatment increased the relative conductivity of cells, and leakage of nucleic acids and proteins further supported the disinfection impact. Additionally, the growth and toxicity of surviving spores after treatment were also greatly reduced. When DBD-CAP was applied to disinfecting dried jujube, the spore number exhibited a 2.67 log cfu/g reduction after treatment without significant damage observed onto the quality ( > ).

中文翻译：

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DBD冷等离子体对干枣黑曲霉的杀菌效果

本研究调查了介质阻挡放电冷气氛等离子体 (DBD-CAP) 对灭活 () 孢子的杀菌效率和作用机制。还对以干枣为加工应用对象的消毒效果和品质进行了研究。结果表明，Weibull + Tail模型在不同处理时间、不同电压下的孢子灭活曲线表现较好，处理15 min后，70 kV的孢子悬浮液中孢子细胞减少了4.05 log（cfu/mL）。扫描电子显微镜（SEM）和透射电子显微镜（TEM）图像进一步支持了这种消毒作用，显示DBD-CAP处理后细胞膜的完整性被破坏，细胞内内容物泄漏。处理过程中活性氧（ROS）水平升高增加了细胞的相对电导率，核酸和蛋白质的泄漏进一步支持了消毒效果。此外，处理后存活孢子的生长和毒性也大大降低。当使用DBD-CAP对干枣进行消毒时，处理后孢子数减少了2.67 log cfu/g，但未观察到对品质的显着损害(>)。