Lipid droplets, as the centre of lipid metabolism, interact with the endoplasmic reticulum, mitochondria, lysosome, nucleus and other organelles to maintain the body's energy balance through vesicle transport or membrane contact. Disruption of the interaction process will lead to the development of lipid metabolism-related diseases. In recent years, many fluorescent probes have been developed to visually follow the dynamic interaction process visually between lipid droplets and other organelles. Existing reviews on lipid droplets fluorescent probes are mainly categorised and reviewed in terms of the emission band of probes, mother fluorophores and design strategies. However, probes that focus on the interactions between lipid droplets and other organelles can provide further insight into the whole process from lipid droplets formation to transformation as well as the mechanisms of diseases related to lipid metabolism. This paper mainly reviews the progress of fluorescent probes targeting lipid droplets interactions with other organelles in the last five years and is divided into dual-targeted and multi-targeted sections based on the number of targeted organelles. The focus is on the biological contacts and dynamic interaction processes between lipid droplet-centred organelles observed by fluorescence imaging with dual-targeted and multi-targeted probes in the article. The targeting groups and reaction sites of the probes are marked in the figure. Moreover, the paper briefly describes the structure and biological functions of lipid droplets and their connections with other organelles. Perhaps in addition to the imaging performance of the probes, we believe that the use of precision instruments to provide high spatio-temporal resolution and reliable results is also a key factor in observational studies. Finally, we summarize the development prospect of multi-color multi-targeted fluorescent probes related to lipid droplets. We expect to bring novel thinking to the design of lipid droplet fluorescent probes and provide new ideas for exploring diagnostic and therapeutic options for lipid droplet-related diseases.

中文翻译：

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荧光探针 - 阐明脂滴和其他细胞器之间的相互作用网络

脂滴作为脂质代谢的中心，通过囊泡运输或膜接触与内质网、线粒体、溶酶体、细胞核等细胞器相互作用，维持机体的能量平衡。相互作用过程的破坏将导致脂质代谢相关疾病的发生。近年来，人们开发了许多荧光探针来直观地跟踪脂滴与其他细胞器之间的动态相互作用过程。现有脂滴荧光探针的综述主要从探针的发射带、母荧光团和设计策略等方面进行分类和综述。然而，关注脂滴与其他细胞器之间相互作用的探针可以进一步深入了解脂滴形成到转化的整个过程以及脂质代谢相关疾病的机制。本文主要综述了近五年来针对脂滴与其他细胞器相互作用的荧光探针的研究进展，并根据靶向细胞器的数量分为双靶点和多靶点部分。文章重点研究了双靶点和多靶点探针荧光成像观察到的以脂滴为中心的细胞器之间的生物接触和动态相互作用过程。图中标出了探针的靶向基团和反应位点。此外，论文还简要描述了脂滴的结构和生物学功能及其与其他细胞器的联系。也许除了探头的成像性能外，我们认为使用精密仪器提供高时空分辨率和可靠的结果也是观测研究的关键因素。最后总结了脂滴相关多色多靶点荧光探针的发展前景。我们期望为脂滴荧光探针的设计带来新的思路，为探索脂滴相关疾病的诊断和治疗方案提供新思路。