Introduction:: Lung carcinoma presents an aggressive evolution, with its carriers having reduced survival. Late diagnosis is one of the main factors of death. In the neoplasia in question, there is an established correlation with increases in Alpha-Fetoprotein (AFP) serum concentrations. Method:: Commonly used diagnostic methods are invasive or inaccessible. Therefore, a low-cost, non-invasive method would be extremely promising, and biomarkers can be used to achieve this goal. Electrochemical biosensors are a promising approach for detecting analytes of clinical interest using innovative bioreceptors. In this work, we obtained an electrochemical biosensor based on a hybrid ligand metal-organic structure (ZIF-8-905%) and functionalized carbon nanotubes (MWCNTs- COOH) in association with the lectin Concanavalin A (ConA), as a biorecognition element for detecting AFP in human serum from patients with lung carcinoma. Cyclic Voltammetry (CV), Square Wave Voltammetry (SWV), and Electrochemical Impedance Spectroscopy (EIS) were used to characterize the development of this biosensor. Microscopic analysis through Atomic Force Microscopy (AFM) revealed the formation of ConA-AFP complexes, pointing out the sensor's ability to identify the target analyte. Result:: The blocking electron transfer effect in the electrode-redox pair interface assessed AFP detection. The ZIF-8-905%/MWCNTs-COOH/ConA platform exhibited a limit of detection (LOD) of 7.98 ng/mL, and a limit of quantification (LOQ) of 23.78ng/mL was also estimated. In addition, the biosensor showed excellent selectivity towards interfering biomolecules. Conclusion:: Therefore, the biosensor represents an efficient form of detection, contributing to research that aims to detect tumor biomarkers and ensure better prognoses.

中文翻译：

---

基于 Zif-8-905% 金属有机纳米复合材料和碳纳米管的伴刀豆球蛋白 a 检测甲胎蛋白的生物传感器

简介:: 肺癌呈现出侵袭性的演变，其携带者的生存率降低。晚期诊断是死亡的主要因素之一。在所讨论的肿瘤中，与甲胎蛋白（AFP）血清浓度的增加存在确定的相关性。方法:: 常用的诊断方法是侵入性的或难以接近的。因此，一种低成本、非侵入性的方法将是非常有前途的，并且生物标志物可以用来实现这一目标。电化学生物传感器是使用创新生物受体检测临床感兴趣的分析物的一种有前景的方法。在这项工作中，我们获得了一种基于混合配体金属有机结构 (ZIF-8-905%) 和功能化碳纳米管 (MWCNTs-COOH) 的电化学生物传感器，与凝集素伴刀豆球蛋白 A (ConA) 结合，作为生物识别元件用于检测肺癌患者血清中的 AFP。使用循环伏安法（CV）、方波伏安法（SWV）和电化学阻抗谱（EIS）来表征该生物传感器的开发。通过原子力显微镜 (AFM) 进行的显微分析揭示了 ConA-AFP 复合物的形成，表明传感器具有识别目标分析物的能力。结果::电极-氧化还原对界面的电子传递阻断效应评估了AFP的检测。 ZIF-8-905%/MWCNTs-COOH/ConA 平台的检测限 (LOD) 为 7.98 ng/mL，估计定量限 (LOQ) 为 23.78 ng/mL。此外，生物传感器对干扰生物分子表现出优异的选择性。结论：因此，生物传感器代表了一种有效的检测形式，有助于旨在检测肿瘤生物标志物并确保更好的预后的研究。