ObjectiveIn this study, we analyzed and investigated the antioxidant, anti-inflammatory, and antitumoral properties of methanolic (Met), dichloromethane (Dic), ethyl acetate (Ac), and diethyl ether (Et) fruit extracts of Ammodaucus leucotrichus on the MCF-7 breast cancer cell line.MethodsThe extracts of Ammodaucus leucotrichus fruits, explored in this study, were obtained by cold maceration and analyzed by LC-MS. 1,1-diphenyl-2-picrylhydrazyl (DPPH), β-Carotene and Bovine Serum Albumin (BSA) assays were used to assess antioxidant and anti-inflammatory activities. The antiproliferative and proapoptotic effects of extracts were determined by the Resazurin test and quantitative real-time polymerase chain reaction (qRT-PCR) analysis.ResultsPhytochemical screening of Ammodaucus leucotrichus fruit extracts revealed the presence of flavonoids (luteolin and cynaroside), iridoid derivatives, phenolic acids, and identified Rhodojaponin—a major cytoxic compound. The DPPH and BSA assays revealed the most potent radical-scavenging activity (IC50 = 0.16 mg/mL) and anti-inflammatory effect (IC50 = 35.87 μg/mL) in Met extract. Met, Dic, Ac, and Et extracts, at 100 µg/mL, exhibited significant antiproliferative activity against MCF-7 reaching inhibition percentage of 34%, 31%, 28%, and 13%, respectively. qRT-PCR analysis was marked by the inhibition of proliferative and inflammatory markers Proliferating Cell Nuclear Antigen (PCNA) and IL-6 and the up-regulation of antioxidant and proapoptotic genes Nrf2, Bax, and Caspase 3 in Met and Dic extracts, without significant changes to anti-apoptotic gene Bcl-2 expression.ConclusionThe study revealed the potential therapeutic effects of Ammodaucus leucotrichus fruit extracts, suggesting they could be used as an adjuvant to breast cancer therapies.

中文翻译：

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Ammodaucus leucotrichus Coss 的化学特征和抗氧化、抗炎和抗增殖作用的评估。 & 持续时间。水果提取物对人类乳腺癌的作用

目的在本研究中，我们分析和研究了 Ammodaucus leucotrichus 的甲醇（Met）、二氯甲烷（Dic）、乙酸乙酯（Ac）和乙醚（Et）果实提取物在 MCF- 上的抗氧化、抗炎和抗肿瘤特性。 7乳腺癌细胞系。方法本研究探索的白毛蝽果实提取物通过冷浸渍获得，并进行LC-MS分析。使用 1,1-二苯基-2-三硝基苯肼 (DPPH)、β-胡萝卜素和牛血清白蛋白 (BSA) 测定来评估抗氧化和抗炎活性。通过刃天青测试和定量实时聚合酶链式反应 (qRT-PCR) 分析确定提取物的抗增殖和促凋亡作用。 结果 Ammodaucus leucotrichus 果实提取物的植物化学筛选显示其含有黄酮类化合物（木犀草素和西葫芦苷）、环烯醚萜衍生物、酚类物质。酸，并鉴定出Rhodojaponin——一种主要的细胞毒性化合物。 DPPH 和 BSA 测定揭示了最有效的自由基清除活性（IC50= 0.16 mg/mL) 和抗炎作用 (IC50= 35.87 μg/mL) 在甲硫氨酸提取物中。 100 µg/mL 的 Met、Dic、Ac 和 Et 提取物对 MCF-7 表现出显着的抗增殖活性，抑制百分比分别为 34%、31%、28% 和 13%。 qRT-PCR 分析的特点是 Met 和 Dic 提取物中增殖和炎症标志物增殖细胞核抗原 (PCNA) 和 IL-6 受到抑制，抗氧化和促凋亡基因 Nrf2、Bax 和 Caspase 3 上调，但没有显着影响。抗凋亡基因 Bcl-2 表达的变化。结论该研究揭示了 Ammodaucus leucotrichus 果实提取物的潜在治疗作用，表明它们可以用作乳腺癌治疗的佐剂。