Currently available silver-based antiseptic wound dressings have limited patient effectiveness. There exists a need for wound dressings that behave as comfortable degradable hydrogels with a strong antibiotic potential. The objectives of this project were to investigate the combined use of gallates (either epi gallo catechin gallate (EGCG), Tannic acid, or Quercetin) as both PVA crosslinking agents and as potential synergistic antibiotics in combination with silver nanoparticles. Crosslinking was assessed gravimetrically, silver and gallate release was measured using inductively coupled plasma and HPLC methods, respectively. Synergy was measured using 96-well plate FICI methods and in-gel antibacterial effects were measured using planktonic CFU assays. All gallates crosslinked PVA with optimal extended swelling obtained using EGCG or Quercetin at 14% loadings (100 mg in 500 mg PVA with glycerol). All three gallates were synergistic in combination with silver nanoparticles against both gram-positive and -negative bacteria. In PVA hydrogel films, silver nanoparticles with EGCG or Quercetin more effectively inhibited bacterial growth in CFU counts over 24 h as compared to films containing single agents. These biocompatible natural-product antibiotics, EGCG or Quercetin, may play a dual role of providing stable PVA hydrogel films and a powerful synergistic antibiotic effect in combination with silver nanoparticles.

中文翻译：

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没食子酸酯交联 PVA 水凝胶膜中含没食子酸酯化合物与银纳米粒子的协同抗菌作用

目前可用的银基抗菌伤口敷料对患者的疗效有限。需要一种具有强大抗生素潜力的舒适可降解水凝胶的伤口敷料。该项目的目标是研究没食子酸酯（表没食子儿茶素没食子酸酯 (EGCG)、单宁酸或槲皮素）作为 PVA 交联剂以及与银纳米粒子组合作为潜在协同抗生素的组合用途。通过重量分析评估交联，分别使用感应耦合等离子体和高效液相色谱法测量银和没食子酸盐的释放。使用 96 孔板 FICI 方法测量协同作用，并使用浮游 CFU 测定测量凝胶内抗菌效果。所有没食子酸酯交联 PVA 均采用 EGCG 或槲皮素以 14% 负载量（100 mg 溶于 500 mg PVA 和甘油）获得，具有最佳的延长溶胀效果。所有三种没食子酸酯与银纳米粒子结合对革兰氏阳性和阴性细菌均具有协同作用。在 PVA 水凝胶薄膜中，与含有单一试剂的薄膜相比，含有 EGCG 或槲皮素的银纳米粒子在 24 小时内更有效地抑制 CFU 计数中的细菌生长。这些生物相容性天然产物抗生素，EGCG 或槲皮素，可以发挥双重作用，提供稳定的 PVA 水凝胶薄膜，并与银纳米颗粒结合产生强大的协同抗生素效应。