Accurate identification of dopamine (DA), uric acid (UA), and acetaminophen (APAP) is critical to safeguarding human health. However, their simultaneous detection is still challenging due to their similar oxidation potentials. In this work, a series of new composites based on cerium-organic framework (Ce-MOF) and carbon black (CB) were constructed by one-pot method at room temperature. The introduction of CB can significantly affect the conductivity and specific surface area of the composites. The optimal composite Ce-MOF-COOH/CB can identify DA, UA, and APAP simultaneously with low detection limit of 0.030, 0.045, and 0.057 μM and wide detection range of 2.5–310.0, 3.0–305.0, and 2.5–617.0 μM, respectively. Furthermore, density-functional theory (DFT) calculation was performed to explore the influence of functional groups (-NH or –COOH) on the sensing performance. It was found that the introduced functional groups can obviously enhance the host-guest interactions through the formation of strong hydrogen bonds, which is beneficial for promoting sensing performance. In addition, the constructed electrode could also detect DA, UA, and APAP in real samples, which indicated its potential for practical applications. This study provides a feasible approach to utilize MOFs as electrochemical sensors for simultaneously detection of biomolecules.

中文翻译：

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一锅法合成铈-有机骨架@炭黑复合材料并同时检测多巴胺、尿酸和对乙酰氨基酚

准确鉴定多巴胺（DA）、尿酸（UA）和对乙酰氨基酚（APAP）对于保障人类健康至关重要。然而，由于它们相似的氧化电位，它们的同时检测仍然具有挑战性。在这项工作中，通过一锅法在室温下构建了一系列基于铈有机骨架（Ce-MOF）和炭黑（CB）的新型复合材料。 CB的引入可以显着影响复合材料的电导率和比表面积。最佳复合Ce-MOF-COOH/CB可以同时识别DA、UA和APAP，检测限低至0.030、0.045和0.057 μM，检测范围宽至2.5–310.0、3.0–305.0和2.5–617.0 μM。分别。此外，还进行了密度泛函理论（DFT）计算，以探讨官能团（-NH或-COOH）对传感性能的影响。研究发现，引入的官能团可以通过形成强氢键明显增强主客体相互作用，有利于提升传感性能。此外，构建的电极还可以检测实际样品中的DA、UA和APAP，这表明了其实际应用的潜力。这项研究提供了一种利用MOF作为电化学传感器同时检测生物分子的可行方法。